Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3738
標題: 神經導管以多醣改質及其奈米粒對周邊神經再生之影響
The modification of nerve conduits by a polysaccharide or its nanopartices and the effect on peripheral nerve regeneration
作者: 翁志宗
Weng, Chih-Tsung
關鍵字: poly(D, L-lactide);聚乳酸;atmospheric air plasma;grafing;tremella fuciformis;nanoparticles;大氣電漿;接枝;銀耳多醣;奈米粒子
出版社: 化學工程學系所
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摘要: 
本研究探討使用非對稱孔洞之生物可降解材料聚乳酸(PLA)為基材,利用大氣電漿改質接枝具生物相容性之銀耳多醣,以掃描式電子顯微鏡、化學分析光譜儀等表面分析評估改質結果。第二部分則利用銀耳多醣與幾丁聚醣來製備銀耳多醣-幾丁聚醣水膠奈米粒子,並以穿透式電子顯微鏡(TEM)、動態光散射(DLS)分析及動態電泳儀探討其粒徑、表面電位及其降解特性。體外實驗進行小鼠神經幹細胞與大鼠神經膠細胞結果顯示其增生與基因表現量皆有明顯上升,銀耳多醣粒子也能於適當之濃度下讓細胞有較佳的活性表現。在大鼠坐骨神經創傷的修復上功能性分析與組織學染色研究結果顯示,表面接枝銀耳多醣之神經導管或內填充銀耳奈米粒之神經導管其修復情形皆優於控制組;而此導管再搭配神經幹細胞治療大鼠坐骨神經損傷,可進一步促進神經再生。

The biodegradable poly(D, L-lactide) (PLA) substrates with asymmetric microporosity were grafted with Tremella fuciformis (TF) in this study after the atmospheric air plasma treatment. The surface of the modified substrates was characterized by the scanning electron microscopy (SEM) and attenuated total reflection Forrier-Transformed infrared (ATR-FTIR) spectroscopy. Hydrogel nanoparticles from TF and chitosan were also prepared. The nanoparticles were characterized by the transmission electron microscopy (TEM), dynamic light scattering (DLS) and electrophoretic light scattering (ELS). The TF modified surface and TF nanoparticles were found to enhance cell proliferation and gene expression of neural stem cells (NSC) and glial cell line C6 in vitro. The conduits modified by TF and those filled with TF nanoparticles were tested for their ability to repair the gap defect of rat sciatic nerve. Results showed that both strategies enhanced the functional recovery and axon regeneration of the nerve, especially for the conduits modified by TF. When the neural stem cells were seeded onto the conduits, the nerve regeneration was further enhanced.
URI: http://hdl.handle.net/11455/3738
其他識別: U0005-1308200919494200
Appears in Collections:化學工程學系所

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