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|標題:||The Infection of Chicken Tracheal Epithelial Cells with a H6N1 Avian Influenza Virus||作者:||Shen, Ching-I
|關鍵字:||tracheal epithelial cell;influenza virus;sialic acid;viral receptor||Project:||Plos One, Volume 6, Issue 5, Page(s) e18894.||摘要:||
Sialic acids (SAs) linked to galactose (Gal) in alpha 2,3- and alpha 2,6-configurations are the receptors for avian and human influenza viruses, respectively. We demonstrate that chicken tracheal ciliated cells express alpha 2,3-linked SA, while goblet cells mainly express alpha 2,6-linked SA. In addition, the plant lectin MAL-II, but not MAA/MAL-I, is bound to the surface of goblet cells, suggesting that SA2,3-linked oligosaccharides with Gal beta 1-3GalNAc subterminal residues are specifically present on the goblet cells. Moreover, both alpha 2,3- and alpha 2,6-linked SAs are detected on single tracheal basal cells. At a low multiplicity of infection (MOI) avian influenza virus H6N1 is exclusively detected in the ciliated cells, suggesting that the ciliated cell is the major target cell of the H6N1 virus. At a MOI of 1, ciliated, goblet and basal cells are all permissive to the AIV infection. This result clearly elucidates the receptor distribution for the avian influenza virus among chicken tracheal epithelial cells and illustrates a primary cell model for evaluating the cell tropisms of respiratory viruses in poultry.
|Appears in Collections:||生命科學系所|
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