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標題: Characterization of Axon Formation in the Embryonic Stem Cell-Derived Motoneuron
作者: Pan, Hung-Chuan
Wu, Ya-Ting
Shen, Shih-Cheng
Wang, Chi-Chung
Tsai, Ming-Shiun
Cheng, Fu-Chou
Lin, Shinn-Zong
Chen, Ching-Wen
Liu, Ching-San
Su, Hong-Lin
關鍵字: Embryonic stem cell;Axon formation;Motoneuron;Neural differentiation
Project: Cell Transplantation, Volume 20, Issue 4, Page(s) 493-502.
The developing neural cell must form a highly organized architecture to properly receive and transmit nerve signals. Neural formation from embryonic stem (ES) cells provides a novel system for studying axonogenesis, which are orchestrated by polarity-regulating molecules. Here the ES-derived motoneurons, identified by HB9 promoter-driven green fluorescent protein (GFP) expression, showed characteristics of motoneuron-specific gene expression. In the majority of motoneurons, one of the bilateral neurites developed into an axon that featured with axonal markers, including Tau I, vesicle acetylcholine transporter, and synaptophysin. Interestingly, one third of the motoneurons developed bi-axonal processes but no multiple axonal GFP cell was found. The neuronal polarity-regulating proteins, including the phosphorylated AKT and ERK, were compartmentalized into both of the bilateral axonal tips. Importantly, this aberrant axon morphology was still present after the engraftment of GFP(+) neurons into the spinal cord, suggesting that even a mature neural environment fails to provide a proper niche to guide normal axon formation. These findings underscore the necessity for evaluating the morphogenesis and functionality of neurons before the clinical trials using ES or somatic stem cells.
ISSN: 0963-6897
DOI: 10.3727/096368910x536464
Appears in Collections:生命科學系所

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