Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37698
DC FieldValueLanguage
dc.contributor.authorLu, Y.Z.en_US
dc.contributor.author葛其梅zh_TW
dc.contributor.authorLin, C.H.en_US
dc.contributor.authorCheng, F.C.en_US
dc.contributor.authorHsueh, C.M.en_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T08:00:00Z-
dc.date.available2014-06-06T08:00:00Z-
dc.identifier.issn0304-3940zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/37698-
dc.description.abstractMicroglia-derived protection of brain cells (microglia, astrocytes, and neurons) during in vitro ischemic stress (deprivation of glucose, oxygen, and serum) was determined. Trypan blue exclusion assay, immunoblocking assay, Western blot analysis. and ELISA assay were used to determine the molecular mechanisms responsible for the microglia-derived protection. Results demonstrated that supermatants from the ischemic microglia protected all three cell-types from ischemia-induced damage by releasing the transforming growth factor-beta1 (TGF-beta1) and glial cell line-derived neurotrophic factor (GDNF). The protection of microglia was TGF-beta1 related, whereas astrocytes protection was GDNF-dependent. The protection of neurons was TGF-beta1 and GDNF independent. and the molecular nature responsible for their protection remains to be determined. These results indicate contribution from the surrounding cells and the types of receptors expressed on different brain cells probably also play an important role in determining their fate against ischemia. (C) 2004 Elsevier Ireland Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationNeuroscience Lettersen_US
dc.relation.ispartofseriesNeuroscience Letters, Volume 373, Issue 2, Page(s) 159-164.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.neulet.2004.10.004en_US
dc.subjectin vitro ishchemiaen_US
dc.subjectmicrogliaen_US
dc.subjectastrocyteen_US
dc.subjectneuronen_US
dc.subjectGDNFen_US
dc.subjectTGB-beta 1en_US
dc.subjectmiddle cerebral-arteryen_US
dc.subjecttransforming growth-factor-beta-1en_US
dc.subjectgdnfen_US
dc.subjectexpressionen_US
dc.subjectbeta-sen_US
dc.subjecttransienten_US
dc.subjectocclusionen_US
dc.subjecttgf-beta-1en_US
dc.subjectsurvivalen_US
dc.subjectinjuryen_US
dc.subjectdegenerationen_US
dc.titleMolecular mechanisms responsible for microglia-derived protection of Sprague-Dawley rat brain cells during in vitro ischemiaen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.neulet.2004.10.004zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生命科學系所
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