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標題: Isoform expression of Na+-K+-ATPase alpha-subunit in gills of the teleost Oreochromis mossambicus
作者: Lee, T.H.
Tsai, J.C.
Fang, M.J.
Yu, M.J.
Hwang, P.P.
關鍵字: chloride cell;sodium pump;ion regulation;branchial chloride cells;euryhaline teleost;anguilla-anguilla;na,k-atpase;tissue;isozymes;localization;sequence;salinity;na+,k+-atpase
Project: American Journal of Physiology-Regulatory Integrative and Comparative Physiology
期刊/報告no:: American Journal of Physiology-Regulatory Integrative and Comparative Physiology, Volume 275, Issue 3, Page(s) R926-R932.
Three isoform-specific antibodies, 6F against the alpha(1)-isoform of the avian sodium pump, HERED against the rat alpha(2)-isoform, and Ax2 against the rat alpha(3)-isoform, were used to detect the expression of Na+-K+-ATPase alpha-subunits in gills of a teleost, the tilapia (Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for alpha(1)- and alpha(3)-sofoonms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of alpha(1)-like isoform alone and consequently the ratio of alpha(1)-like to alpha(3)-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for alpha(1)- and alpha(3)-isoforms. alpha(1)-like and alpha(3)-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia.
ISSN: 0363-6119
Appears in Collections:生命科學系所

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