The relationship between 'deep-hole' mitochondria-rich cells and salinity adaptation in the euryhaline teleost, Oreochromis mossambicus

Abstract

The present study elucidates the relationship between deep-hole MR cells (Lee et al. 1996) and salinity adaptation in tilapia (Oreochromis mossambicus). Freshwater tilapia were transferred to salt water of various salinities, i.e., 5 (hypotonic), 10 (isotonic), 20 (hypertonic), and 30 parts per thousand (hypertonic), for 2 weeks. The density of MR cells, protein content, activity, and localization of the sodium pump were examined. There was no significant difference in serum osmolarity and Na+, K+, Cl- levels in fish of the various treatment groups. The amounts of protein and activity of Na,K-ATPase were elevated in fish from SW with the highest salinity. MR cells observed by scanning electron microscope revealed small pits (0.5-1.0 mum in diameter) in groups from hypotonic and isotonic water and large crypts (2.4-3.8 mum in diameter) in fish from hypertonic water. Moreover, the density of these deep-hole MR cells increased significantly in fish adapted to hypertonic SW. Larger and more numerous deep-hole MR cells of euryhaline tilapia may account for higher protein amounts and activities of Na,K-ATPase, probably to meet the physiological demand of euryhaline teleosts engaged in hyporegulation.