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標題: Visfatin-induced expression of inflammatory mediators in human endothelial cells through the NF-kappa B pathway
作者: Lee, W.J.
赫, 林
Wu, C.S.
Lin, H.
Lee, I.T.
Wu, C.M.
Tseng, J.J.
Chou, M.M.
Sheu, W.H.H.
關鍵字: visfatin;endothelial cell;inflammation;adhesion;type-2 diabetes-mellitus;colony-enhancing factor;coronary-heart-disease;adhesion molecules;adipose-tissue;mmp-2/9;production;plasma visfatin;atherosclerosis;obesity;activation
Project: International Journal of Obesity
期刊/報告no:: International Journal of Obesity, Volume 33, Issue 4, Page(s) 465-472.
Background: Visfatin is an adipokine that is highly expressed in visceral fat. Plasma levels of visfatin have been reported to be higher in subjects with obesity and/or type 2 diabetes mellitus. However, the role of visfatin in endothelial dysfunction has been largely unexplored. Objectives: We investigated the possible pathogenic role of visfatin in endothelial dysfunction, particularly focusing on its effect on inflammatory mediators. Design: Primary human umbilical vein endothelial cells (HUVECs) pretreated with visfatin ( 1, 10 and 50 ng ml(-1)) were used to study the relationship between visfatin and endothelium dysfunction. Expressions of adhesion molecules (ICAM-1, VCAM-1 and E-selectin) and cytokines ( interleukin (IL)-6 and IL-8) affected by visfatin were investigated by enzyme-linked immunosorbent assay, flow cytometry and real-time PCR. Activity of nuclear factor (NF)-kappa B was examined by electrophoretic mobility shift assay. Results: At a visfatin concentration of 50 ng ml(-1), significant increases in IL-6, IL-8, ICAM-1, VCAM-1 and E-selectin gene expression along with increased IL-6, IL-8 and sE-selectin protein levels in the conditioned medium were detected. Flow cytometry showed that the addition of visfatin significantly increased ICAM-1 expression and VCAM-1 expression ( 10 and 50 ng ml(-1), respectively). Electrophoretic mobility shift assay confirmed that visfatin increased the DNA-binding activity of NF-kappa B. In addition, pretreatment with visfatin ( 10 and 50 ng ml(-1)) increased human monocyte cell line THP-1 adhesion to HUVECs. Conclusions: Our findings suggest that visfatin causes endothelial dysfunction by increasing inflammatory and adhesion molecule expression at least partly through the upregulation of NF-kappa B activity.
ISSN: 0307-0565
DOI: 10.1038/ijo.2009.24
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