Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3792
標題: 比較thiol及dithiol monolayer修飾網版印刷電極於電容型免疫感測器上檢測人血清白蛋白
Comparison of thiol and dithiol monolayer modification on screen-printed electrode in capacitive immunosensor for detection of human serum albumin
作者: 張建偉
Chang, Chen-Wei
關鍵字: Capacitive immunosensor;電容型免疫感測器;Human serum albumin;Self-assembled monolayer;Gold nanoparticles;label-free;人類血清白蛋白;自組裝單分子膜;奈米金;label-free
出版社: 化學工程學系所
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摘要: 
本研究利用自組裝單層薄膜吸附上奈米金粒子於網版印刷電極(screen-printed electrode)上,製備一新型電容免疫感測器;當形成抗體抗原複合物時會造成電容值的改變,因此透過量測電容值的改變即可檢測樣品的濃度,將之應用於檢測人血清白蛋白(Human serum albumin,HSA)上。實驗中探討最適化製備電容型免疫感測器的條件:抗體固定濃度為0.5 mg/ml;抗原結合pH值為pH 7.4;抗體抗原結合時間為50分鐘;量測電容時電解質濃度為10 mM。
研究中首先比較thiourea和cysteamin兩者於電容型免疫感測器上之效益,由結果可得知cysteamine其效果較佳。另外開發一個新的策略,將cysteamine SAM上的胺基和cysteamine所具之硫醇基透過glutaraldehyde形成雙硫醇分子膜,然後比較單硫醇分子膜和雙硫醇分子膜對奈米金粒子的吸附效果在免疫感測器的檢測上。透過最適化條件,其cysteamine所製備之單硫醇分子膜感測器檢測HSA線性範圍在0.78~800 ng/ml,而cysteamine所製備之雙硫醇分子膜感測器檢測HSA線性範圍在0.39~1000 ng/ml。因此開發一個新的雙硫醇分子膜應用於免疫感測器上,能有效提升奈米金粒子吸附抗體的量,增大其檢測範圍與極限。
此電容型免疫感測器在檢測過程中不須加入標記(label-free),檢測快速(real-time),並可應用於單次使用、拋棄式的感測器上,並於臨床檢驗分析上檢測HSA。

Fabrication of a novel capacitive immunosensor based on self-assembled monolayer (SAM) incorporated with gold nanoparticles on a screen-printed thick-film electrode was the first time built for the detection of human serum albumin (HSA). Anti-HSA was immobilized on gold nanoparticles incorporated to a self-assembled monolayer onto the gold working electrode. Interaction of anti-HSA and HSA was directly detected by the capacitance measurement. The optimal preparation condition for fabricating this HSA immunosensor was studied. The optimized manufacturing reaction conditions were as follows: anti-HSA concentration of 0.5 mg/ml, pH 7.4 immuno binding time of 50 minutes, and the electrolyte concentration of 10 mM.
Both thiourea and cysteamine were used to build the capacitive immunosensor. Cysteamine has a better detection range and detection limit. Second. The dithiol monolayer was compared with the thiol monolayer in the immunosensor detection. Experimental results showed that the immunosensor made by cysteamine responded linearly to HSA concentration. The linear detection range for thiol monolayer was in the range of 0.78-800 ng/ml (R=0.994) and dithiol monolayer was in the range of 0.39-1000 ng/ml (R=0.9974). The dithiol monolayer in immunosensor with gold nanoparticles to absorb more antibody displayed an increase in the detection range and detection limit.
The capacitive immunosensor is a label-free and real time detection. In the assessment of the potential applications, the developed sensor can be employed for the single use, disposable biosensor in the clinical analysis of HSA.
URI: http://hdl.handle.net/11455/3792
其他識別: U0005-0408201022254500
Appears in Collections:化學工程學系所

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