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標題: A water-based triphenyltetrazolium chloride method for the evaluation of green plant tissue viability
作者: Lin, C.H.
Chen, B.S.
Yu, C.W.
Chiang, S.W.
關鍵字: 2,3,5-triphenyltetrazolium chloride;triphenylformazan;viability;waxapple;Syzygium samarangense;2,3,5-triphenyltetrazolium chloride;indicator;reduction
Project: Phytochemical Analysis
期刊/報告no:: Phytochemical Analysis, Volume 12, Issue 3, Page(s) 211-213.
A water-based 2,3,5-triphenyltetrazolium chloride (TTC) partitioning method using n-hexane for the evaluation of green plant tissue viability has been developed. Conventionally, the reduction of TTC to insoluble red-coloured triphenylformazan (TPF) has been used to detect seed, bud, leaf and cultured cell viability. However, the 95% ethanol used to extract TPF also extracts various pigments, such as chlorophyll, from plant tissues which interfere with the absorption of TPF at 485 nm. This new water-based method improves upon the current method by eliminating the interfering pigments in the hexane layer, and by minimising the non-enzymatic oxidation of the sample. When used to evaluate the tissue viability of chill-stressed waxapple (Syzygium samarangense) plants, the refined method had a greater sensitivity than the electrolyte leakage method and thus provided a more precise assessment for the physiological state of various plant tissues. Copyright (C) 2001 John Wiley & Sons, Ltd.
ISSN: 0958-0344
DOI: 10.1002/pca.570
Appears in Collections:生命科學系所

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