Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38077
DC FieldValueLanguage
dc.contributor.authorLiao, Y.F.en_US
dc.contributor.author洪慧芝zh_TW
dc.contributor.authorHsieh, H.C.en_US
dc.contributor.authorLiu, G.Y.en_US
dc.contributor.authorHung, H.C.en_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T08:00:29Z-
dc.date.available2014-06-06T08:00:29Z-
dc.identifier.issn0003-2697zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38077-
dc.description.abstractA simple, continuous spectrophotometric assay for peptidylarginine deiminase (PAD) is described. Deimination of peptidylarginine results in the formation of peptidylcitrulline and ammonia. The ammonia released during peptidylarginine hydrolysis is coupled to the glutamate-dehydrogenase-catalyzed reductive amination of of alpha-ketoglutarate to glutamate and reduced nicotinamide adenine dinucleotide (NADH) oxidation. The disappearance of absorbance at 340 nm due to NADH oxidation is continuously measured. The specific activity obtained by this new protocol for highly purified human PAD is comparable to that obtained by a commonly used colorimetric procedure, which measures the ureido group of peptidylcitrulline by coupling with diacetyl monoxime. The present continuous spectrophotometric method is highly sensitive and accurate and is thus suitable for enzyme kinetic analysis of PAD. The Ca2+ concentration for half-maximal activity of PAD obtained by this method is comparable to that previously obtained by the colorimetric procedure. (c) 2005 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationAnalytical Biochemistryen_US
dc.relation.ispartofseriesAnalytical Biochemistry, Volume 347, Issue 2, Page(s) 176-181.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.ab.2005.09.027en_US
dc.subjectpeptidylarginine deiminaseen_US
dc.subjectglutamate-dehydrogenaseen_US
dc.subjectspectrophotometricen_US
dc.subjectcontinuous assayen_US
dc.subjectgene organizationen_US
dc.subjectexpression analysisen_US
dc.subjectarginine deiminaseen_US
dc.subjectmolecular-cloningen_US
dc.subjectcdna cloningen_US
dc.subjecthuman skinen_US
dc.subjectperformanceen_US
dc.subjecthydrolysisen_US
dc.subjectcitrullineen_US
dc.subjectenzymesen_US
dc.titleA continuous spectrophotometric assay method for peptidylarginine deiminase type 4 activityen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.ab.2005.09.027zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:生命科學系所
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