Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3809
標題: 原位感溫型水膠之製備與其於蛋白質藥物傳輸之應用
In-situ thermo-sensitive hydrogel as protein drug delivery.
作者: 李昶均
Li, Chang-Chun
關鍵字: AvastinR;癌思停;thermo-sensitive;block copolymers;hydrogels;溫度敏感性;團聯共聚物;智慧型水膠
出版社: 化學工程學系所
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摘要: 
藥物開發於近年來進入新的里程碑,在人類基因解碼後,人們逐漸了解到致病因子,開發更具有專一性的藥物。即是以特殊抗原、生長因子受體、血管新生因子來做為藥物的標的,而合成出蛋白質藥物。這種具有專一性的藥物在治療過程中可以避免傷害人體正常的細胞,但是蛋白質很脆弱容易受到環境變化而使其失活失去該有的作用。其半衰期通常都不長,所以當接受治療後需要頻繁地施打。藥物施打常採用靜脈注射或口服,在進入循環系統後容易受到破壞。所以尋找載體來保護藥物並直接送到病灶,亦可以降低藥物所需的劑量。
故本研究以合成生物可降解性的溫感性水膠聚㗁唑啉-聚己內之酯-聚㗁唑啉,並以Avastin作為本研究之模型藥物(model drug)以探討水膠對於蛋白質藥物之傳輸及定點釋放之可行性。而Avastin近年來應用於眼睛黃斑部病變之治療,具有相當的療效,所以本研究採用人類視網膜色素上皮細胞ARPE-19作為毒性測試之模型,以不同體積之水膠進行試驗,其結果顯示此細胞的存活率皆在95%以上,顯示出此材料良好的生物相容性。並此溫感性水膠在包覆Avastin後,其釋放曲線為零級釋放,對於維持體內藥物濃度具有相當良好的效果。顯示出此水膠對於蛋白質藥物的傳輸具有相當的潛力。

Drug development in recent years entered a new milestone in human genome decoding, people gradually learned that virulence factors, development of more specific drugs. That is specific antigen, growth factor receptors, an giogenic factors are to do as the subject of drugs, and the synthesis of protein drugs. This drug has a specific course of treatment to avoid damage in normal human cells, but protein is facile deactivation when environmental changes. The drug usually has a short half-life, so it has frequent injections. Drugs commonly used intravenous injections or orally, after entering the circulatory system vulnerable to damage. So finding for carriers to protect the drugs and directly to the lesion, also can reduce the dose of drug required.
This study we use the thermo-sensitive hydrogel to protect the protein, and it can undergoing sol - gel transition with the temperature increase. It can be mixed with the drugs for injection, and then in-situ forming gels for sustain release.
Biocompatibility of the material, because this study used a drug Avastin for the eye in recent years, the treatment of macular degeneration, so ARPE cell toxicity tests using the model. Different volume placed on the cell, the results show that ECE hydrogel has good biocompatibility for ARPE cells.
URI: http://hdl.handle.net/11455/3809
其他識別: U0005-1210201011133600
Appears in Collections:化學工程學系所

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