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標題: 以聚乙烯醇修飾PET不織布做為固定化金屬螯合親和吸附材之研究
Preparation of polyvinyl alcohol-coated PET non-woven fabric as immobilized metal ion affinity adsorbents
作者: 邱美雪
Chiu, Mei-Hsueh
關鍵字: PET non-woven fabric;PET不織布;Borax;RSM;Protein adsorption;硼砂;反應曲面法;蛋白質吸附
出版社: 化學工程學系所
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本研究以PET不織布當作固定化金屬親和層析法的固體載體,藉由高分子聚乙烯醇(Polyvinyl alcohol,PVA)與交聯劑硼砂 (Sodium tetraborate,Borax)交聯反應產生的鍵結,在不織布表面形成三維的網狀結構後,接著利用化學合成的方式接上活化劑、螯合劑和金屬離子後, 即完成固定化金屬親和螯合吸附材之製備。同時,本研究將利用反應曲面法(Response Surface Methodology, RSM),以PVA濃度、活化劑濃度和活化時間做為影響銅離子量之因子,探討各因子對銅離子鍵結量之影響,找出最適極值的區域。
從實驗結果發現不織布經過改質後,其PVA鍵結量可達90%而PET不織布含水率從未改質前的52%提升至75%。表面形態觀察(SEM) 也可觀察出改質前後的差異。當參數條件設為 PVA濃度6%、活化劑濃度 30% 和活化時間 20hr時,銅離子鍵結量可達78 mmol/m2。經由回應曲面法得知,銅離子鍵結量與設定之三個因子(PVA濃度、活化劑濃度、活化時間)存在密切關聯性,同時也證明實驗結果的準確性與此反應曲面的預測模型具有可靠性。最後,利用製備出的吸附基材進行蛋白質吸附實驗發現,製備出的吸附基材具有吸附蛋白質的功能,但是在製備固體載體時要注意PVA濃度不可以太高,否則會將孔洞阻塞,使其失去吸附蛋白質的功能。

In this study, polyvinyl alcohol-coated PET non-woven fabrics were prepared by crosslinking PVA with Borax onto the surface of PET non-woven fabrics, yielding a three-dimensional network structure. The PVA-coated PET non-woven fabrics were then activated by epoxidation, coupled with IDA (chelating agent), and loaded with Cu2+ to attain an immobilized metal affinity (IMA) absorbents. The effect of PVA concentration, activating agent concentration and activation time on the amount of chelated metal ion were analyzed by RSM (Response Surface Methodology) to decide the optimum values of independent factors for the best response value.
The results showed that the PET non-woven fabrics modified with molecular weight 90,000 of polyvinyl alcohol had mass ratio 90%, while the swelling ratio increased from 52% to 75%. The PVA-coated PET non-woven fabrics were easily distinguished from uncoated and coated preparation through SEM. It was found that when PVA concentration 6%, activating agent concentration 30% and, activation time 20hr, the amount of immobilized Cu2+ reached 78 mmol/m2. The results from RSM reveal the reliability for this study. Afterward, we show that the immobilized metal affinity (IMA) absorbents have the ability of. However, it was found that with increasing the concentration of PVA caused a block behavior on absorbents. Hence, it is necessary to keep a suitable porous size, or it will easy to lose the utility of protein adsorption from the prepared absorbents.
其他識別: U0005-1808201010573700
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