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標題: Lactoferrin as a Natural Regimen for Selective Decontamination of the Digestive Tract: Recombinant Porcine Lactoferrin Expressed in the Milk of Transgenic Mice Protects Neonates from Pathogenic Challenge in the Gastrointestinal Tract
作者: 陳全木
Yen, C.C.
Lin, C.Y.
Chong, K.Y.
Tsai, T.C.
Shen, C.J.
Lin, M.F.
Su, C.Y.
Chen, H.L.
Chen, C.M.
關鍵字: bovine lactoferrin;escherichia-coli;listeria-monocytogenes;candida-albicans;pichia-pastoris;infection;combination;prevention;piglets;cells
Project: Journal of Infectious Diseases
期刊/報告no:: Journal of Infectious Diseases, Volume 199, Issue 4, Page(s) 590-U164.
Background. Nosocomial infection with antibiotic-resistant strains is a major threat to critical care medicine. Selective decontamination of the digestive tract (SDD) is one of the strategies used to reduce ventilator-associated pneumonia and sepsis in critically ill patients. In the present study, we performed pathogenic challenges of the digestive tract in a transgenic milk-fed animal model to test whether porcine lactoferrin (pLF) is an effective SDD regimen. Methods. Transgenic mice expressing recombinant pLF in their milk at a mean +/- SD concentration of 120 +/- 13.6 mg/L during the lactation stage fed normal CD-1 mice pups for 4 weeks. The pups were subsequently challenged with pathogenic Escherichia coli, Staphylococcus aureus, and Candida albicans. Results. Compared with the control groups fed wild-type (normal) milk, the groups fed pLF-enriched milk demonstrated statistically significant improvements in weight gain; lower bacterial numbers in intestinal fluid, blood, and liver; healthier microvilli in the small intestine; and alveoli in the lungs. Conclusions. Our results showed that oral administration of pLF-enriched milk to mice led to broad-spectrum antimicrobial activity in the digestive tract and protected the mucosa of the small intestine from injury, implying that pLF can be used as an effective SDD regimen.
ISSN: 0022-1899
DOI: 10.1086/596212
Appears in Collections:生命科學系所

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