Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38278
DC FieldValueLanguage
dc.contributor.author陳全木zh_TW
dc.contributor.authorChen, C.M.en_US
dc.contributor.authorHu, C.L.en_US
dc.contributor.authorWang, C.H.en_US
dc.contributor.authorHung, C.M.en_US
dc.contributor.authorWu, H.K.en_US
dc.contributor.authorChoo, K.B.en_US
dc.contributor.authorCheng, W.T.K.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-06T08:00:40Z-
dc.date.available2014-06-06T08:00:40Z-
dc.identifier.issn1040-452Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38278-
dc.description.abstractA sensitive technique for the sexing of bovine embryos was developed using polymerase chain reaction (PCR) amplification of the bovine amelogenin (bAML) gene on the X- and Y-chromosomes of Holstein dairy cattle. Cloning and DNA sequencing showed a 45.1% homology between the fifth intron of the bAML-X and bAML-Y gene with multiple deletions. A pair of sex-specific primers was designed to allow amplification of a single fragment of 467-bp from the X-chromosome of female cattle and two fragments of 467-bp and 341-bp from the X- and Y-chromosomes of male cattle. The primers were successfully applied to bovine sexing from single blastomeres isolated from day-6 to day-7 cow embryos by direct cell lysis and PCR. Our protocol of embryo sexing should be applicable to the diagnosis of defective genes in vitro in human embryos and in other domestic or recreational animals. (C) 1999 Wiley-Liss, Inc.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular Reproduction and Developmenten_US
dc.relation.ispartofseriesMolecular Reproduction and Development, Volume 54, Issue 3, Page(s) 209-214.en_US
dc.relation.uri2-yen_US
dc.relation.urihttp://dx.doi.org/10.1002/(sici)1098-2795(199911)54:3<209::aid-mrd1>3.3.coen_US
dc.subjectgender determinationen_US
dc.subjectbovine blastomereen_US
dc.subjectamelogenin geneen_US
dc.subjectsingle-cellen_US
dc.subjectPCRen_US
dc.subjectregion encodesen_US
dc.subjecty-chromosomeen_US
dc.subjectdna-sequenceen_US
dc.subjectembryosen_US
dc.subjectidentificationen_US
dc.subjectheterogeneityen_US
dc.subjectgoatsen_US
dc.subjectzfyen_US
dc.titleGender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin geneen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1002/(sici)1098-2795(199911)54:3<209::aid-mrd1>3.3.co;2-yzh_TW
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生命科學系所
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