Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38282
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dc.contributor.authorChen, C.M.en_US
dc.contributor.author陳全木zh_TW
dc.contributor.authorWang, C.H.en_US
dc.contributor.authorWu, S.C.en_US
dc.contributor.authorLin, C.C.en_US
dc.contributor.authorLin, S.H.en_US
dc.contributor.authorCheng, W.T.K.en_US
dc.date2002zh_TW
dc.date.accessioned2014-06-06T08:00:40Z-
dc.date.available2014-06-06T08:00:40Z-
dc.identifier.issn0962-8819zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38282-
dc.description.abstractHemophilia A is one of the major inherited bleeding disorders caused by a deficiency or abnormality in coagulation factor VIII (FVIII). Hemophiliacs have been treated with whole plasma or purified FVIII concentrates. The risk of transmitting blood-borne viruses and the cost of highly purified FVIII are the major factors that restrict prophylaxis in hemophilia therapy. One of the challenges created by the biotechnology revolution is the development of methods for the economical production of highly purified proteins in large scales. Recent developments indicate that manipulating milk composition using transgenesis has focused mainly on the mammary gland as a bioreactor to produce pharmaceuticals. In the present study, a hybrid gene containing bovine alpha-lactalbumin and human FVIII cDNA was constructed for microinjection into the pronuclei of newly fertilized mouse eggs. The alphaLA-hFVIII hybrid gene was confirmed to be successfully integrated and stably germ-line transmitted in 12 (seven females/five males) lines. Western-blot analysis of milk samples obtained from eight of the transgenic founders and F1 offspring indicated that the recombinant hFVIII was secreted into the milk of the transgenic mice. The concentrations of rFVIII ranged from 7.0 to 50.2 mug/ml, over 35-200-fold higher than that in normal human plasma. Up to 13.4 U/ml of rFVIII was detected in an assay in which rFVIII restored normal clotting activity to FVIII-deficient human plasma.en_US
dc.language.isoen_USzh_TW
dc.relationTransgenic Researchen_US
dc.relation.ispartofseriesTransgenic Research, Volume 11, Issue 3, Page(s) 257-268.en_US
dc.relation.urihttp://dx.doi.org/10.1023/a:1015651302674en_US
dc.subjectalpha-lactalbumin promoteren_US
dc.subjecthemophilia Aen_US
dc.subjecthuman factor VIIIen_US
dc.subjectmammaryen_US
dc.subjectglanden_US
dc.subjecttransgenic animalen_US
dc.subjectclotting factor-viiien_US
dc.subjectprotein-productionen_US
dc.subjectskin fibroblastsen_US
dc.subjectcodingen_US
dc.subjectregionen_US
dc.subjectgene-therapyen_US
dc.subjectglanden_US
dc.subjectsheepen_US
dc.subjectcdnaen_US
dc.subjecthemophiliaen_US
dc.subjectdeletionsen_US
dc.titleTemporal and spatial expression of biologically active human factor VIII in the milk of transgenic mice driven by mammary-specific bovine alpha-lactalbumin regulation sequencesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1023/a:1015651302674zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextno fulltext-
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