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標題: Clostridium strain co-cultures for biohydrogen production enhancement from condensed molasses fermentation solubles
作者: Hsiao, C.L.
Chang, J.J.
Wu, J.H.
Chin, W.C.
Wen, F.S.
Huang, C.C.
Chen, C.C.
Lin, C.Y.
關鍵字: Biohydrogen;Molasses;Clostridium;Hydrogenase;hydraulic retention time;hydrogen-production;waste-water;acidaminococcus fermentans;anaerobic-bacteria;sewage-sludge;microflora;sucrose;system;feasibility
Project: International Journal of Hydrogen Energy
期刊/報告no:: International Journal of Hydrogen Energy, Volume 34, Issue 17, Page(s) 7173-7181.
An anaerobic continuous-flow hydrogen fermentor was operated at a hydraulic retention time of 8 h using condensed molasses fermentation solubles (CMS) substrate of 40 g-COD/L. Serum bottles were used for seed micro-flora cultivation and batch hydrogen fermentation tests (CMS substrate concentrations of 10-160 g-COD/L). Three hydrogen-producing bacterial strains Clostridium sporosphaeroides F52, Clostridium tyrobutyricum F4 and Clostridium pasteurianum F40 were isolated from the seed fermentor and used as the seeding microbes in single and mixed-culture cultivations for determining their hydrogen productivity. These strains possessed specific hydrogenase genes that could be detected from CMS-fed hydrogen fermentors and were major hydrogen producers. C. pasteurianum F40 was the dominant strain with a high hydrogen production rate while C. sporosphaeroides F52 may play a main role in degrading carbohydrate and glutamate. These strains could be co-cultivated as a symbiotic mixed-culture process to enhance hydrogen productivity. C. pasteurianum F40 or C. tyrobutyricum F4 co-culture with the glutamate-utilizing strain C. sporosphaeroides F52 efficiently enhanced hydrogen production by 12-220% depending on the substrate CMS concentrations. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
ISSN: 0360-3199
DOI: 10.1016/j.ijhydene.2009.06.028
Appears in Collections:生命科學系所

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