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標題: Expressing a bacterial mercuric ion binding protein in plant for phytoremediation of heavy metals
作者: Hsieh, J.L.
Chen, C.Y.
Chiu, M.H.
Chein, M.F.
Chang, J.S.
Endo, G.
Huang, C.C.
關鍵字: Biosorption;Gram-positive bacterial MerP;Heavy metals;Mercuric ion;binding protein;Phytoremediation;class-ii transposon;arabidopsis-thaliana;organomercurial compounds;resistance determinants;minamata bay;merp;transformation;genes;agrobacterium;diversity
Project: Journal of Hazardous Materials
期刊/報告no:: Journal of Hazardous Materials, Volume 161, Issue 2-3, Page(s) 920-925.
A specific mercuric ion binding protein (MerP) originating from transposon TnMERI1 of Bacillus megaterium strain MB1 isolated from Minamata Bay displayed good adsorption capability for a variety of heavy metals. In this study, the Gram-positive MerP protein was expressed in transgenic Arabidopsis to create a model system for phytoremediation of heavy metals. Under control of an actin promoter, the transgenic Arabidpsis showed higher tolerance and accumulation capacity for mercury, cadium and lead when compared with the control plant. Results from confocal microscopy analysis also indicate that MerP was localized at the cell membrane and vesicles of plant cells. The developed transgenic plants possessing excellent meta1-accumulative ability could have potential applications in decontamination of heavy metals. (C) 2008 Elsevier B.V. All rights reserved.
ISSN: 0304-3894
DOI: 10.1016/j.jhazmat.2008.04.079
Appears in Collections:生命科學系所

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