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標題: Splicing of a Bacterial Group II Intron from Bacillus megaterium Is Independent of Intron-Encoded Protein
作者: Chien, M.F.
Tosa, S.
Huang, C.C.
Endo, G.
關鍵字: group II intron;intron-encoded protein;splicing;maturase binding;rna;domain;identification;diversity;sediment;genes;ai2;dna
Project: Microbes and Environments
期刊/報告no:: Microbes and Environments, Volume 24, Issue 1, Page(s) 28-32.
The splicing of a bacterial group II subclass B intron from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of An in vivo assay showed that the splicing occurred in the absence of IEP. An in vitro assay showed that was spliced under conditions similar to those of the intracellular environment with no help from other biological molecules. Because all group II introns previously reported needed IEPs for their splicing in vivo, our results suggest that is an "actual" self-splicing group II intron. This is also the first report to recognize the existence of group II introns that independently splice mRNA in vivo. The self-splicing of a bacterial intron may support that eukaryotic spliceosomal introns originated in bacterial genomes.
ISSN: 1342-6311
DOI: 10.1264/jsme2.ME08540
Appears in Collections:生命科學系所

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