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|標題:||The adsorption protein genes of Xanthomonas campestris filamentous phages determining host specificity||作者:||Lin, N.T.
|關鍵字:||gram-negative bacteria;single-stranded-dna;phi-lf;nucleotide-sequence;escherichia-coli;pv campestris;deletion;mutagenesis;coat protein;iii protein;genome||Project:||Journal of Bacteriology||期刊/報告no：:||Journal of Bacteriology, Volume 181, Issue 8, Page(s) 2465-2471.||摘要:||
Gene III (gIII) of phi Lf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determined, resulting in the revision of the translation initiation site from the one previously predicted for this gene. For comparative study, the gm of phi Xv, a filamentous phage specifically infecting X. campestris pv. vesicatoria, was cloned and sequenced. The deduced amino acid sequences of these two pIIIs exhibit a high degree of identity in their C-terminal halves and possess the structural features typical of the adsorption proteins of filamentous phages: a signal sequence in the N terminus, a long glycine-rich region near the center, and a hydrophobic membrane anchorage domain in the C terminus. The regions between gIII and the upstream gVIII, 128 nucleotides in both phages, are larger than those of other filamentous phages. A hybrid phage of phi Xv, consisting of the phi Lf pIII and all the other components derived from phi Xv, was able to infect X. campestris pv. campestris but not X. campestris pv, vesicatoria, indicating that gill is the gene specifying host specificity and demonstrating the interchangeability of the pIIIs.
|Appears in Collections:||生命科學系所|
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