Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38336
DC FieldValueLanguage
dc.contributor.authorLin, C.H.en_US
dc.contributor.author葛其梅zh_TW
dc.contributor.authorLu, Y.Z.en_US
dc.contributor.authorCheng, F.C.en_US
dc.contributor.authorChu, L.F.en_US
dc.contributor.authorHsueh, C.M.en_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T08:00:43Z-
dc.date.available2014-06-06T08:00:43Z-
dc.identifier.issn0304-3940zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38336-
dc.description.abstractAn in vitro ischemia model was used to determine the molecular mechanisms responsible for the ischemia-induced neuronal cell death. Additionally, the neuronal protective mechanisms of anti-apoptotic drugs against ischemia were also evaluated. In this study, the primary neuronal cultures were incubated in an anoxic chamber with 95% of N-2 and 5% of CO2 for various times. The death rate, degree of the apoptotic damage, reduction of mitochondrial membrane potential, translocation of Bax, release of cytochrome C and activation of caspase-9 and -3 were determined at each time point. Results showed that a Bax-regulated mitochondria- mediated apoptosis is responsible for the in vitro ischemia-induced neuronal death. Reduction in mitochondrial membrane potential plays no role in triggering this apoptosis. Furthermore, the anti-apoptotic drugs: furosemide (a Bax blocker) and ZVAD-fmk (caspase inhibitor) but not cyclosporine A (a NIPT pore blocker), significantly protected the neurons against ischemia-induced damage. This provides an additional consideration in the future selection of new anti-ischemic drugs. (C) 2005 Elsevier Ireland Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationNeuroscience Lettersen_US
dc.relation.ispartofseriesNeuroscience Letters, Volume 387, Issue 1, Page(s) 22-27.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.neulet.2005.06.070en_US
dc.subjectin vitro ischemiaen_US
dc.subjectneuronal cell deathen_US
dc.subjectapoptosisen_US
dc.subjectmitochondriaen_US
dc.subjectBaxen_US
dc.subjectcaspasesen_US
dc.subjectfurosemideen_US
dc.subjectZVAD-fmken_US
dc.subjectpermeability transitionen_US
dc.subjectcytochrome-cen_US
dc.subjectmembraneen_US
dc.subjectdamageen_US
dc.subjectactivationen_US
dc.subjectmechanismsen_US
dc.subjectnecrosisen_US
dc.subjectchannelen_US
dc.titleBax-regulated mitochondria-mediated apoptosis is responsible for the in vitro ischemia induced neuronal cell death of Sprague Dawley raten_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.neulet.2005.06.070zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生命科學系所
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