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標題: 神經幹細胞以小體積微粒球培養對其分化能力之影響
The Behavior of Neural Stem Cells in Alginate Microspheres
作者: 林大強
Lin, Ta-Chiang
關鍵字: alginate;褐藻膠;microspheres;neural stem cells;neurotrophic factors;nerve differentiation;微球體;神經幹細胞;流變儀;神經滋養因子;神經分化
出版社: 化學工程學系所
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本研究是以生物可降解材料-褐藻膠做為基材,以導流針、震動馬達、高頻率撞針系統三種方法去製作微球體,並於製程中包覆神經幹細胞,改善噴霧法包覆效率不高的問題後討論神經幹細胞在微球體內部的生長與活性維持,並分析其基因表現。經過五天的培養後,其神經幹細胞增生率約為140%,之後細胞數約為定值。在生化功能部分,神經生長因子等的基因表現,初期 (3天、5天)相較培養於平面時的表現為佳,而到達較長時間 (14天)時,則發現細胞有聚集的情形,且GFAP表現變強,推測為不同M/G比例與流變儀測出的不同硬度的褐藻膠會對包覆的神經幹細胞有不同的分化效果。

Alginate microspheres were fabricated into microspheres in this study. By three special devices designed in-house, we were able to encapsulate the neural stem cells in calcium chloride crosslinked alginate microspheres. The efficiency of encapsulation could reach about 98%. Neural stem cells transfected by promoter F1B-green fluorescence protein (F1B-GFP reporter) plasmid showed green fluorescence expression in alginate microspheres, indicated that they were undifferentiated and alive. The cell number in alginate microspheres increased 1.4 times after 5 days. The expression level of neurotrophic genes was higher for cells cultured in alginate microspheres than that on TCPS at 3 and 5 days. 3D culture in alginate microspheres promoted the GFAP gene expression at 14 days. The tendency was more pronounced in softer alginate microspheres.
其他識別: U0005-2008201016185500
Appears in Collections:化學工程學系所

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