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標題: Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage
作者: Lee, G.A.
Lin, C.H.
Jiang, H.H.
Chao, H.J.
Wu, C.L.
Hsueh, C.M.
關鍵字: in vitro ischemia;microglia;astrocyte;glial cell line-derived;neurotrophic factor;brain;neurons;gdnf;expression;survival;injury;occlusion;receptors;promotes
Project: Neuroscience Letters
期刊/報告no:: Neuroscience Letters, Volume 356, Issue 2, Page(s) 111-114.
The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
ISSN: 0304-3940
DOI: 10.1016/j.neulet.2003.11.030
Appears in Collections:生命科學系所

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