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標題: Binding potency of peptide fragments of type 1 collagen cross-linked N-telopeptide measured by an enzyme-linked immunosorbant assay
作者: Lung, F.D.T.
Chen, C.H.
Chen, H.Y.
Liou, C.C.
Liou, Y.M.
關鍵字: osteoporosis;cross-linked N-telopeptide;type I collagen;synthetic;overlapping peptides;solid phase peptide synthesis;high performance;liquid chromatography;enzyme-linked immunosorbent assay;bone-resorption;i collagen;postmenopausal women;biochemical markers;alendronate;immunoassay;turnover;serum;urine
Project: Protein and Peptide Letters
期刊/報告no:: Protein and Peptide Letters, Volume 9, Issue 5, Page(s) 451-457.
Osteoporosis represents a major healthcare problem affecting elderly person. Urinary level of the cross-linked N-telopeptide of type I collagen is a sensitive marker of bone resorption. Ten overlapping peptides covering the N-telopeptide of alpha-2 type I collagen were synthesized, purified, and assayed for their relative binding response to anti-type I collagen cross-linked N-telopeptide (NTX) antibody by using a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). Peptides 1, 2, and 3, containing the N-terminal sequence of N-telopeptide, showed higher binding potency than peptides 4-10, suggesting that these peptides may contain binding sites for anti-NTX antibodies, and can serve as the lead for further preparation of their antibodies in order to develop novel bioassays for monitoring the bone loss in humans.
ISSN: 0929-8665
DOI: 10.2174/0929866023408544
Appears in Collections:生命科學系所

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