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|標題:||Binding potency of peptide fragments of type 1 collagen cross-linked N-telopeptide measured by an enzyme-linked immunosorbant assay||作者:||Lung, F.D.T.
|關鍵字:||osteoporosis;cross-linked N-telopeptide;type I collagen;synthetic;overlapping peptides;solid phase peptide synthesis;high performance;liquid chromatography;enzyme-linked immunosorbent assay;bone-resorption;i collagen;postmenopausal women;biochemical markers;alendronate;immunoassay;turnover;serum;urine||Project:||Protein and Peptide Letters||期刊/報告no：:||Protein and Peptide Letters, Volume 9, Issue 5, Page(s) 451-457.||摘要:||
Osteoporosis represents a major healthcare problem affecting elderly person. Urinary level of the cross-linked N-telopeptide of type I collagen is a sensitive marker of bone resorption. Ten overlapping peptides covering the N-telopeptide of alpha-2 type I collagen were synthesized, purified, and assayed for their relative binding response to anti-type I collagen cross-linked N-telopeptide (NTX) antibody by using a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). Peptides 1, 2, and 3, containing the N-terminal sequence of N-telopeptide, showed higher binding potency than peptides 4-10, suggesting that these peptides may contain binding sites for anti-NTX antibodies, and can serve as the lead for further preparation of their antibodies in order to develop novel bioassays for monitoring the bone loss in humans.
|Appears in Collections:||生命科學系所|
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