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標題: Differentially expressed genes after hyper- and hypo-salt stress in the halophilic archaeon Methanohalophilus portucalensis
作者: Shih, C.J.
Lai, M.C.
關鍵字: salt stress response;salt stress adaptation;differential display;RT-PCR;halophilic methanogen;molecular chaperone ClpB;universal;stress protein USPA;methanogenesis;stringent response;saccharomyces-cerevisiae;escherichia-coli;glycine betaine;mismatch;repair;messenger-rna;dna-damage;mycobacterium-tuberculosis;methanosarcina-barkeri;compatible solutes;oxidative stress
Project: Canadian Journal of Microbiology
期刊/報告no:: Canadian Journal of Microbiology, Volume 56, Issue 4, Page(s) 295-307.
Methanohalophilus portucalensis FDF1 can grow over a range of external NaCl concentrations, from 1.2 to 2.9 mol/L. Differential gene expression in response to long-term hyper-salt stress (3.1 mol/L of NaCl) and hypo-salt stress (0.9 mol/L of NaCl) were compared by differential display RT-PCR. Fourteen differentially expressed genes responding to long-term hyper- or hypo-salt stress were detected, cloned, and sequenced. Several of the differentially expressed genes were related to the unique energy-acquiring methanogenesis pathway in this organism, including the transmembrane protein MttP, cobalamin biosynthesis protein, methenyl-H4MPT cyclohydrolase and monomethylamine methyltransferase. One signal transduction histidine kinase was identified from the hyper-salt stress cultures. Moreover, 3 known stress-response gene homologues the DNA mismatch repair protein, MutS, the universal stress protein, UspA, and a member of the protein-disaggregating multichaperone system, ClpB - were also detected. The transcriptional analysis of these long-term salt stress response and adaptation-related genes for cells immediately after salt stress indicated that the expression of the energy metabolism genes was arrested during hyper-salt shock, while the chaperone clpB gene was stimulated by both hypo- and hyper-salt shock.
ISSN: 0008-4166
DOI: 10.1139/w10-008
Appears in Collections:生命科學系所

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