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標題: Characterization of Methanosarcina mazei N2M9705 isolated from an aquaculture fishpond
作者: Lai, M.C.
Shu, C.M.
Chiou, M.S.
Hong, T.Y.
Chuang, M.J.
Hua, J.J.
關鍵字: methanogenic bacteria;methane
Project: Current Microbiology
期刊/報告no:: Current Microbiology, Volume 39, Issue 2, Page(s) 79-84.
A new methanogenic isolate, designated as strain N2M9705 (=OCM 668), was isolated from an aquaculture fishpond near Wang-gong, Taiwan. This strain grew on trimethylamine and methanol, but it did not catabolize H-2-CO2, acetate, or formate. The cells were stained Gram-negative, nonmotile, irregular coccus 0.6-0.8 mu m in diameter. Gas vacuoles were observed and cell aggregated to form various sizes of granules. Cells grew optimally at 32 degrees-37 degrees C with 1% NaCl. The pH range of growth was 6.2-7.4, and higher pH inhibited the cell growth. The cells grew well in minimal medium, but growth was greatly stimulated by yeast extract and peptone. A comparison of 16S rDNA sequences of this organism phylogenetically related to Methanosarcina mazei. This is the first report of methyltrophic methanogenic isolated from an aquaculture fishpond.
ISSN: 0343-8651
DOI: 10.1007/s002849900422
Appears in Collections:生命科學系所

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