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|標題:||Sugar uptake by photomixotrophic soybean suspension cultures||作者:||Yen, H.E.
|關鍵字:||acid invertase;glycine max;photomixotrophic suspension culture;sugar;uptake;sucrose-binding-protein;photosynthetic characteristics;mesembryanthemum-crystallinum;lycopersicon-esculentum;phosphate;synthase;cell-cultures;expression;protoplasts;growth;fruit||Project:||Botanical Bulletin of Academia Sinica||期刊/報告no：:||Botanical Bulletin of Academia Sinica, Volume 40, Issue 2, Page(s) 147-152.||摘要:||
A photomixotrophic soybean (Glycine max) culture with substantial chlorophyll content was used to study the ability of cells to grow on different carbon sources. With the exception of sorbitol, these photomixotrophic cells were able to grow on media containing 3% starch, or 2% sucrose, maltose, glucose and fructose; and the growth curves and biomass production of hexose- or disaccharide-grown cells were all fairly similar. The highest biomass production was found in the sucrose-grown cells. Levels of soluble protein and total chlorophyll and the Chi a/b ratio were highest in the starch-grown cells. Although the growth curves of sucrose-, glucose-, or fructose-grown cells were quite similar, the abilities of cells grown on one source to utilize various carbon sources were quite distinct. C-14-labelling experiments showed that the highest incorporation rates for sucrose and hexoses all occurred during the logarithmic phase with sucrose-grown cells preferentially taking up sucrose and hexose-grown cells preferentially taking up hexoses. Cells maintained on 3% starch showed an ability to take up both sucrose and hexoses with the highest uptake rate when fed with C-14-glucose. The rate of uptake of sucrose was decreased more than twofold as cells entered the stationary phase, while there was a twofold increase in the activity of extracellular acid invertase (EC 18.104.22.168). The clear correlation between low acid invertase activity and high levels of incorporated radioactivity from C-14-sucrose strongly suggested that intact sucrose is being taken up in sucrose-fed soybean cells during the vigorous growth stage, a stage similar to developing soybean pods.
|Appears in Collections:||生命科學系所|
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