Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3845
DC FieldValueLanguage
dc.contributor吳宗明zh_TW
dc.contributor廖建勛zh_TW
dc.contributor.advisor蔡毓楨zh_TW
dc.contributor.author詹筱翎zh_TW
dc.contributor.authorChan, Hsiao-Lingen_US
dc.contributor.other中興大學zh_TW
dc.date2011zh_TW
dc.date.accessioned2014-06-06T05:32:54Z-
dc.date.available2014-06-06T05:32:54Z-
dc.identifierU0005-2506201016573700zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/3845-
dc.description.abstract本研究成功以單股探針DNA(single-stranded DNA, ssDNA, probe DNA),多層壁奈米碳管(multi-walled carbon nanotube, MWNT),幾丁聚醣(chitosan, CHIT),製備出ssDNA/MWNT/CHIT生物奈米複合材料薄膜修飾玻璃碳電極(glassy carbon electrode, GCE),證明具有偵測目標DNA(target DNA)的能力。其中MWNT具有優勢的電化學活性並且成為奈米導線複合材料,CHIT以其優良的成膜性、良好的貼附性成為此次感測器之載體,也以其帶有之NH3+官能基將probe DNA固定於電極上,讓此DNA感測器擁有優秀的線性偵測範圍與不錯的選擇性。此次實驗使用原子力顯微鏡(atomic force microscope, AFM)來確認所製備之生物奈米複合薄膜之表面形態,並觀察其ssDNA在雜交過後之方均根粗糙度(root means square roughness, RMS)改變。為了使該生物感測器之表現最佳化,我們探討幾個實驗條件的最適值如雜交時間、雜交系統之pH值等。本實驗之最佳化後之DNA感測器之靈敏度為84.93(µA/cm2*log mol/L),偵測其target DNA之線性範圍為10-6 M到10-11 M,偵測極限為2×10-12 M。zh_TW
dc.description.abstractA sensitive electrochemical DNA biosensor based on multi-walled carbon nanotubes (MWNT) and chitosan (CHIT) for DNA immobilization and enhanced hybridization detection is presented. The morphology of the resulting MWNT/CHIT/GCE, ssDNA/MWNT/CHIT/GCE and dsDNA/MWNT/CHIT/GCE are investigated by atomic force microscopy (AFM). The decreasing root means square roughness (RMS) shows the probe DNA is immobilized in the MWNT/CHIT film. After hybridization, the increased RMS shows the probe DNA and target DNA hybridized. The hybridization reaction on the electrode was monitor by differential pulse voltammetry (DPV) analysis using K3[Fe(CN)6] as the mediator. The influence of several experimental parameters such as the ratio of the probe DNA to MWNT/CHIT, hybridization time and solution pH value are explored to optimize the electroanalytical performance of the DNA sensor. The linear range of this sensor to target DNA is from 10-6 M to 10-11 M. The detection limit is 210-12 M.en_US
dc.description.tableofcontents總目錄 摘要 i Abstract ii 總目錄 iii 圖目錄 v 第一章 緒論 1 1-1前言 1 1-2分析物簡介及其文獻回顧 2 1-3 生物感測器 6 1-3-1 生物感測器定義 6 1-3-2生物感測器的基本構造與組成 6 1-3-3各式生物感測器之分類與簡介 13 1-4 各式DNA感測器之簡介 15 1-4-1 石英震盪微量天平 16 1-4-2 光學DNA感測器 18 1-4-3 原子力顯微鏡DNA感測器 22 1-4-4 電化學DNA感測器 25 1-5 奈米碳管 40 1-5-1 奈米碳管簡介 40 1-5-2 奈米碳管的特性 42 1-5-3 奈米碳管的應用 43 1-5-4 奈米碳管的分散方法 44 1-6 幾丁聚醣 47 1-6-1 幾丁聚醣的特性 47 1-6-2 幾丁聚醣在感測器上的應用 49 1-7 電化學方法 49 1-7-1 交流阻抗法 51 1-7-2 循環伏安法 54 1-7-3 微分脈衝伏安法 57 1-8 原子力顯微鏡 58 第二章 實驗方法與步驟 60 2-1 實驗藥品 60 2-2 實驗儀器 60 2-3 實驗步驟 61 2-3-1 電極前處理 61 2-3-2 ssDNA/MWNT/CHIT複合薄膜修飾於玻璃碳電極 62 2-3-3 target DNA 雜交及測試 63 第三章 結果與討論 65 3-1 chitosan薄膜修飾GCE(CHIT/GCE)之電化學特性 65 3-2 各薄膜修飾GCE之電化學特性 66 3-3 奈米碳管(MWNT)對DNA雜交之影響 68 3-4 以AFM觀察各種薄膜之表面形態 70 3-5 ssDNA/MWNT/CHIT薄膜浸泡於PBS中之穩定性 73 3-6 CHIT與probe DNA的比例對偵測target DNA之影響 74 3-7 参混入MWNT/CHIT複合薄膜中的probe DNA濃度對偵測target DNA之影響 77 3-8 雜交時間對偵測target DNA之影響 80 3-9 雜交環境之pH值對偵測target DNA之影響 84 3-10 ssDNA/MWNT/CHIT/GCE應用於偵測target DNA 87 3-11 選擇率測試 90 第四章 結論與未來展望 93 4-1 結論 93 4-2 未來展望 93 第五章 文獻參考 95zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2506201016573700en_US
dc.subjectDNA sensoren_US
dc.subjectDNA感測器zh_TW
dc.subjectcarbon nanotubeen_US
dc.subjectchitosanen_US
dc.subject奈米碳管zh_TW
dc.subject幾丁聚醣zh_TW
dc.title多層壁奈米碳管/幾丁聚醣複合薄膜作為電化學DNA感測器之探討zh_TW
dc.titleElectrochemical DNA sensors based on multiwalled carbon nanotubes/chitosan composite filmen_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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