Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38486
DC FieldValueLanguage
dc.contributor.author蘇鴻麟zh_TW
dc.contributor.authorLin, C.D.en_US
dc.contributor.authorLai, C.K.en_US
dc.contributor.authorLin, Y.H.en_US
dc.contributor.authorHsieh, J.T.en_US
dc.contributor.authorSing, Y.T.en_US
dc.contributor.authorChang, Y.C.en_US
dc.contributor.authorChen, K.C.en_US
dc.contributor.authorWang, W.C.en_US
dc.contributor.authorSu, H.L.en_US
dc.contributor.authorLai, C.H.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-06T08:00:51Z-
dc.date.available2014-06-06T08:00:51Z-
dc.identifier.issn0019-9567zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38486-
dc.description.abstractCampylobacter jejuni is a common cause of pediatric diarrhea worldwide. Cytolethal distending toxin, produced by Campylobacter jejuni, is a putative virulence factor that induces cell cycle arrest and apoptosis in eukaryotic cells. Cellular cholesterol, a major component of lipid rafts, has a pivotal role in regulating signaling transduction and protein trafficking as well as pathogen internalization. In this study, we demonstrated that cell intoxication by Campylobacter jejuni cytolethal distending toxin is through the association of cytolethal distending toxin subunits and membrane cholesterol-rich microdomains. Cytolethal distending toxin subunits cofractionated with detergent-resistant membranes, while the distribution reduced upon the depletion of cholesterol, suggesting that cytolethal distending toxin subunits are associated with lipid rafts. The disruption of cholesterol using methyl-beta-cyclodextrin not only reduced the binding activity of cytolethal distending toxin subunits on the cell membrane but also impaired their delivery and attenuated toxin-induced cell cycle arrest. Accordingly, cell intoxication by cytolethal distending toxin was restored by cholesterol replenishment. These findings suggest that membrane cholesterol plays a critical role in the Campylobacter jejuni cytolethal distending toxin-induced pathogenesis of host cells.en_US
dc.language.isoen_USzh_TW
dc.relationInfection and Immunityen_US
dc.relation.ispartofseriesInfection and Immunity, Volume 79, Issue 9, Page(s) 3563-3575.en_US
dc.relation.urihttp://dx.doi.org/10.1128/iai.05175-11en_US
dc.subjecthelicobacter-pylori vacaen_US
dc.subjectintestinal epithelial-cellsen_US
dc.subjectactinobacillus-actinomycetemcomitansen_US
dc.subjectlipid raftsen_US
dc.subjectescherichia-colien_US
dc.subjectcycle arresten_US
dc.subjectsignal-transductionen_US
dc.subjecttumor-suppressoren_US
dc.subjectmembrane domainsen_US
dc.subjectcholera-toxinen_US
dc.titleCholesterol Depletion Reduces Entry of Campylobacter jejuni Cytolethal Distending Toxin and Attenuates Intoxication of Host Cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1128/iai.05175-11zh_TW
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生命科學系所
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.