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標題: The signals of FGFs on the neurogenesis of embryonic stem cells
作者: 蘇鴻麟
Chen, C.W.
Liu, C.S.
Chiu, I.M.
Shen, S.C.
Pan, H.C.
Lee, K.H.
Lin, S.Z.
Su, H.L.
關鍵字: jun nh2-terminal kinase;neural induction;directed differentiation;bmp;inhibition;self-renewal;neurons;mouse;precursors;chick;gastrulation
Project: Journal of Biomedical Science
期刊/報告no:: Journal of Biomedical Science, Volume 17.
Background: Neural induction is a complex process and the detailed mechanism of FGF-induced neurogenesis remains unclear. Methods: By using a serum-free neural induction method, we showed that FGF1 dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells. Results: We demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the rescue of the apoptosis or the enhancement of the proliferation of Sox1(+) cells. We further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein kinase (MAPK), inhibited the neural formation through the inhibition of ES differentiation, but not through the formation of endomesodermal cells. Conclusions: These lines of evidence delineated the roles of FGF downstream signals in the early neural differentiation of ES cells.
ISSN: 1021-7770
DOI: 10.1186/1423-0127-17-33
Appears in Collections:生命科學系所

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