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|標題:||Growth hormone gene polymorphisms and growth performance traits in Duroc, Landrace and Tao-Yuan pigs||作者:||Cheng, W.T.K.
|關鍵字:||gene;growth hormone;RFLP;growth performance;pigs;porcine somatotropin;milk-production;swine;cattle;expression;insulin;breeds||Project:||Theriogenology||期刊/報告no：:||Theriogenology, Volume 54, Issue 8, Page(s) 1225-1237.||摘要:||
This study investigated the restriction fragment length polymorphism (RFLP) of the porcine growth hormone (pGH) gene in Duroc, Landrace, and Tao-Yuan pigs and its effects on growth performance and levels of plasma growth hormone in peripheral circulation. Genomic DNA extracted from 81 Tao-Yuan, 60 Landrace and 48 Duroc pigs were subjected to Southern blot hybridization with a pGH cDNA probe. Polymorphism was detected with the restriction enzymes TaqI and DraI. A comparison of these three breeds showed significant differences in allelic frequencies. Blood samples for radioimmunoassay (RIA) of GH were collected biweekly during the experimental period from pigs 12 to 40 weeks of age. Tao-Yuan pigs showed a mean plasma GH level (2.51 +/- 1.23 ng/mL) that was much lower than that of the Landrace (3.80 +/- 1.52 ng/mL) and Duroc (4.20 +/- 1.03 ng/mL.) pigs (P< 0.05). Moreover, the Tao-Yuan pigs also showed poorer growth performance than the Landrace and the Duroc pigs both in the daily weight gain (0.37 +/- 0.06 vs. 0.67 +/- 0.05 and 0.70 +/- 0.05 kg/day, P< 0.01) and feed efficiency (3.12 +/- 0.28 vs. 2.60 +/- 0.14 and 2.52 +/- 0.12, P< 0.05). These results suggest that the growth performance trait in these pigs is highly correlated with their growth hormone genotype. (C) 2000 by Elsevier Science Inc.
|Appears in Collections:||生命科學系所|
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