Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38551
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dc.contributor.authorCheng, F.C.en_US
dc.contributor.author蘇鴻麟zh_TW
dc.contributor.authorTai, M.H.en_US
dc.contributor.authorSheu, M.L.en_US
dc.contributor.authorChen, C.J.en_US
dc.contributor.authorYang, D.Y.en_US
dc.contributor.authorSu, H.L.en_US
dc.contributor.authorHo, S.P.en_US
dc.contributor.authorLai, S.Z.en_US
dc.contributor.authorPan, H.C.en_US
dc.contributor.author何素鵬zh_TW
dc.contributor.author許美鈴zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:00:55Z-
dc.date.available2014-06-06T08:00:55Z-
dc.identifier.issn0022-3085zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/38551-
dc.description.abstractObject. Human amniotic fluid derived mesenchymal stem cells (AFMSCs) have been shown to promote peripheral nerve regeneration, and the local delivery of neurotrophic factors may additionally enhance nerve regeneration capacity. The present study evaluates whether the transplantation of glia cell line derived neurotrophic factor (GDNF) modified human AFMSCs may enhance regeneration of sciatic nerve after a crush injury. Methods. Peripheral nerve injury was produced in Sprague-Dawley rats by crushing the left sciatic nerve using a vessel clamp. Either GDNF-modified human AFMSCs or human AFMSCs were embedded in Matrigel and delivered to the injured nerve. Motor function and electrophysiological studies were conducted after I and 4 weeks. Early or later nerve regeneration markers were used to evaluate nerve regeneration. The expression of GDNF in the transplanted human AFMSCs and GDNF-modified human AFMSCs was monitored at 7-day intervals. Results. Human AFMSCs were successfully transfected with adenovirus, and a significant amount of GDNF was detected in human AFMSCs or the culture medium supernatant. Increases in the sciatic nerve function index, the compound muscle action potential ratio, conduction latency, and muscle weight were found in the groups treated with human AFMSCs or GDNF-modified human AFMSCs. Importantly, the GDNF-modified human AFMSCs induced the greatest improvement. Expression of markers of early nerve regeneration, such as increased expression of neurofilament and BrdU and reduced Schwann cell apoptosis, as well as late regeneration markers, consisting of reduced vacuole counts, increased expression of Luxol fast blue and S100 protein, paralleled the results of motor function. The expression of GDNF in GDNF-modified human AFMSCs was demonstrated up to 4 weeks; however, the expression decreased over time. Conclusions. The GDNF-modified human AFMSCs appeared to promote nerve regeneration. The consecutive expression of GDNF was demonstrated in GDNF-modified human AFMSCs up to 4 weeks. These findings support a nerve regeneration scenario involving cell transplantation with additional neurotrophic factor secretion. (DOI: 10.3171/2009.8.JNS09850)en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Neurosurgeryen_US
dc.relation.ispartofseriesJournal of Neurosurgery, Volume 112, Issue 4, Page(s) 868-879.en_US
dc.relation.urihttp://dx.doi.org/10.3171/2009.8.jns09850en_US
dc.subjectglia cell line-derived neurotrophic factoren_US
dc.subjectnerve regenerationen_US
dc.subjectamnioticen_US
dc.subjectfluid mesenchymal stem cellen_US
dc.subjectschwann-cellsen_US
dc.subjectperipheral-nerveen_US
dc.subjectspinal-corden_US
dc.subjectfunctional recoveryen_US
dc.subjectgrowth-factoren_US
dc.subjectin-vivoen_US
dc.subjectaxonal regenerationen_US
dc.subjectsurvival factoren_US
dc.subjectmotor-neuronsen_US
dc.subjectgene-transferen_US
dc.titleEnhancement of regeneration with glia cell line-derived neurotrophic factor-transduced human amniotic fluid mesenchymal stem cells after sciatic nerve crush injury Laboratory investigationen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.3171/2009.8.jns09850zh_TW
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
Appears in Collections:生命科學系所
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