Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38574
標題: Differential Differences in Methylation Status of Putative Imprinted Genes among Cloned Swine Genomes
作者: Shen, Chih-Jie
Winston, T.K.Cheng
Wu, Shinn-Chih
Chen, Hsiao-Ling
Tsai, Tung-Chou
Yang, Shang-Hsun
Chen, Chuan-Mu
Project: PLoS ONE, Volume 7, Issue 2
摘要: 
DNA methylation is a major epigenetic modification in the mammalian genome that regulates crucial aspects of gene
function. Mammalian cloning by somatic cell nuclear transfer (SCNT) often results in gestational or neonatal failure with only
a small proportion of manipulated embryos producing live births. Many of the embryos that survive to term later succumb
to a variety of abnormalities that are likely due to inappropriate epigenetic reprogramming. Aberrant methylation patterns
of imprinted genes in cloned cattle and mice have been elucidated, but few reports have analyzed the cloned pig genome.
Four surviving cloned sows that were created by ear fibroblast nuclear transfer, each with a different life span and multiple
organ defects, such as heart defects and bone growth delay, were used as epigenetic study materials. First, we identified
four putative differential methylation regions (DMR) of imprinted genes in the wild-type pig genome, including two
maternally imprinted loci (INS and IGF2) and two paternally imprinted loci (H19 and IGF2R). Aberrant DNA methylation,
either hypermethylation or hypomethylation, commonly appeared in H19 (45% of imprinted loci hypermethylated vs. 30%
hypomethylated), IGF2 (40% vs. 0%), INS (50% vs. 5%), and IGF2R (15% vs. 45%) in multiple tissues from these four cloned
sows compared with wild-type pigs. Our data suggest that aberrant epigenetic modifications occur frequently in the
genome of cloned swine. Even with successful production of cloned swine that avoid prenatal or postnatal death, the
perturbation of methylation in imprinted genes still exists, which may be one of reason for their adult pathologies and short
life. Understanding the aberrant pattern of gene imprinting would permit improvements in future cloning techniques.
URI: http://hdl.handle.net/11455/38574
DOI: 10.1371/journal.pone.0032812
Appears in Collections:生命科學系所

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