Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38582
標題: Endothelial Japanese encephalitis virus infection enhances migration and adhesion of leukocytes to brain microvascular endothelia via MEK-dependent expression of ICAM1 and the CINC and RANTES chemokines
作者: Lai, Ching-Yi
Ou, Yen-Chuan
Chang, Cheng-Yi
Pan, Hung-Chuan
Chang, Chen-Jung
Liao, Su-Lan
Su, Hong-Lin
Chen, Chun-Jung
關鍵字: adhesion molecules;blood–brain barrier;chemo-kines;flavivirus;neuroinflammation
Project: Journal of Neurochemistry, Volume 123, Issue 2, pages 250–261
摘要: 
Currently, the underlying mechanisms and the specific cell types associated with Japanese encephalitis-associated leukocyte trafficking are not understood. Brain microvascular endothelial cells represent a functional barrier and could play key roles in leukocyte central nervous system trafficking. We found that cultured brain microvascular endothelial cells were susceptible to Japanese encephalitis virus (JEV) infection with limited amplification. This type of JEV infection had negligible effects on cell viability and barrier integrity. Instead, JEV-infected endothelial cells attracted more leukocytes adhesion onto surfaces and the supernatants promoted chemotaxis of leukocytes. Infection with JEV was found to elicit the elevated production of intercellular adhesion molecule-1, cytokine-induced neutrophil chemoattractant-1, and regulated-upon-activation normal T-cell expressed and secreted, contributing to the aforementioned leukocyte adhesion and chemotaxis. We further demonstrated that extracellular signal-regulated kinase was a key upstream regulator which stimulated extensive endothelial gene induction by up-regulating cytosolic phospholipase A2, NF-κB, and cAMP response element-binding protein via signals involving phosphorylation. These data suggest that JEV infection could activate brain microvascular endothelial cells and modify their characteristics without compromising the barrier integrity, making them favorable for the recruitment and adhesion of circulating leukocytes, thereby together with other unidentified barrier-disrupting mechanisms contributing to Japanese encephalitis and associated neuroinflammation.
URI: http://hdl.handle.net/11455/38582
DOI: 10.1111/j.1471-4159.2012.07889.x
Appears in Collections:生命科學系所

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