Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/38598
DC FieldValueLanguage
dc.contributor.authorLiu, Jen-Weien_US
dc.contributor.authorHsu, Yi-Chaoen_US
dc.contributor.authorKao, Chien-Yuen_US
dc.contributor.authorSu, Hong-Linen_US
dc.contributor.authorChiu, Ing-Mingen_US
dc.date2012-1-30zh_TW
dc.date.accessioned2014-06-06T08:00:58Z-
dc.date.available2014-06-06T08:00:58Z-
dc.identifier.urihttp://hdl.handle.net/11455/38598-
dc.description.abstractIn regular culture conditions with leukemia inhibitory factor (LIF), the majority of mouse embryonic stem cells (mESCs) are maintained in a self-renewal stage; very few mESCs have differentiated morphology. When LIF is withdrawn, mESCs tend to differentiate; this differentiation process can be enhanced by the introduction of exogenous fibroblast growth factor (FGF). Here, we show that even in the presence of exogenous FGF1, mESCs can maintain self-renewal and expression of pluripotency markers in the presence of LIF. To elucidate the mechanism in which LIF dominates over the FGF1, extracellular signal-regulated kinase 1/2 (Erk1/2) signaling of mESCs cultured in a medium containing FGF1 or LIF/FGF1 was examined. The results demonstrate that Erk1/2 was activated by FGF1 in the absence of LIF; however, the FGF1-induced Erk1/2 phosphorylation was suppressed when LIF was introduced. Moreover, FGF1-Erk1/2 downregulation was inhibited by a signal transducer and activator of the transcription 3 (Stat3) inhibitor WP1066, suggesting that LIF-induced Stat3 activation plays an important role in the FGF1-Erk1/2 inhibition in mESCs. We further demonstrate that the binding affinity of phospho-Erk1/2 and Sprouty2 was increased via Stat3 activation. Binding of phospho-Erk1/2 and Sprouty2 blocks the activation of Erk1/2 signaling, thus inhibiting the downstream differentiation process in mESCs. Our findings demonstrate, for the first time, that LIF-induced Stat3 phosphorylation plays an important role in promoting the binding of phospho-Erk1/2 and Sprouty2, and thus inhibiting FGF-induced differentiation.en_US
dc.language.isoen_USzh_TW
dc.relationStem Cells and Development, Volume 22, Issue 8, Page(s) 1190-1197.en_US
dc.relation.urihttp://dx.doi.org/10.1089/scd.2012.0229en_US
dc.titleLeukemia Inhibitory Factor-Induced Stat3 Signaling Suppresses Fibroblast Growth Factor 1-Induced Erk1/2 Activation to Inhibit the Downstream Differentiation in Mouse Embryonic Stem Cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1089/scd.2012.0229zh_TW
dc.contributor.catalogerWei Chun Wangen_US
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:生命科學系所
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.