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標題: Investigations of the effects of nanostructure and micro horizontal vibration on cell proliferation
作者: 李靜雯
Ching-Wen, LI
關鍵字: vacuum air-extraction process;真空抽氣法;nanostructured;PLGA scaffold;micro horizontal vibrations;BEC;奈米結構;微水平震動;牛頸動脈內皮細胞
出版社: 生醫工程研究所
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Interactions between cell and scaffold material is one of the main interesting topics in tissue engineering. In this research, influences of nanostructured PLGA scaffolds and micro horizontal vibrations on bovine endothelial cells (BEC) were investigated, respectively.
In the study of the interactions between BEC and nanostructured PLGA scaffolds, two simple fabrication methods to fabricate orderly nanostructured PLGA scaffolds using anodic aluminum oxide (AAO) template were conducted. In the vacuum air-extraction approach, the PLGA solution was cast on an AAO template first. The vacuum air-extraction process was then applied to suck the semi-congealed PLGA into the nanopores of the AAO template to form a bamboo sprouts array of PLGA. The surface roughness of the nanostructured scaffolds, ranging from 20 nm to 68 nm, can be controlled by the sucking time of the vacuum air-extraction process. In the replica molding approach, the PLGA solution was cast on the orderly scraggy barrier-layer surface of an AAO membrane to fabricate a PLGA scaffold of concave nanostructure. Cell culture experiments using BEC demonstrated that the nanostructured PLGA membrane can increase the cell growing rate, especially for the bamboo sprouts array scaffolds with smaller surface roughness.
In the study of the influences of micro horizontal vibrations on BEC proliferations, different frequencies 0.5 Hz, 1 Hz, and 2 Hz respectively with a 20 micro-meter amplitude, using a piezoelectric micro-positioning stage, were applied to nanostructured and flat PLGA scaffolds during cell cultivation. It was found that the proliferation of BEC on nanostructured PLGA scaffold under micro horizontal vibrations was suppressed. It is presumed that the nanostructures made the surface of the scaffold more hydrophobic, hence degraded the adhesions between BECs and the scaffold. On the contrary, the proliferations of BECs on a flat PLGA scaffold were enhanced under vibrations of 1 Hz frequency and 20 micro-meter amplitude. The 1 Hz frequency is close to the pulse rate of mature cattle (60-80 Hz).
In this research, both the nanostructured PLGA scaffolds and the 1 Hz micro horizontal vibrations on flat PLGA scaffolds were discovered to be able to enhance the proliferation of BECs. However, the micro horizontal vibrations using a piezoelectric micro-positioning stage is much easier to be implemented.
其他識別: U0005-2107200913440900
Appears in Collections:生醫工程研究所

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