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標題: 動態與靜態場對於脂肪幹細胞之影響
The effect of dynamic and static conditions on the adipose-derived adult stem cells
作者: 許家榮
Hsu, Chia-Jung
關鍵字: 脂肪幹細胞;adipose-derived adult stem cells (ADAS);牛頸動脈內皮細胞;EGF;PDGF-BB;bovine carotid artery endothelial cells (BEC);EGF;PDGF-BB
出版社: 生醫工程研究所
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In this study, human and rat adipose-derived adult stem cells (ADAS) were characterized. Next, the feasibility of endothelial differentiations of human and rat ADAS and growth factors [ epidermal growth factor (EGF) and platelet-derived growth factor (PDGF-BB) ] treated ADAS (ADAS-GFs) cultured under dynamic conditions were examined. For parameter identification, bovine carotid arterial endothelial cells (BEC) were first cultured under dynamic condition. The result showed that cell proliferation was better for BEC when cultured with dynamic strains at 1 Hz. Next, rat ADAS (rADAS) and rat ADAS-GFs (rADAS-GFs) were tested in the same condition. By comparing the expressions of endothelial marker genes from rADAS and rADAS-GFs cultured under steady condition, rADAS-GFs had higher endothelial differentiation ability. When rADAS-GFs were cultured under dynamic condition, the expression of CD31 and CD34 genes were enhanced, which indicated an increase in the endothelial differentiation. Therefore, rADAS-GFs cultured under dynamic condition showed more endothelial differentiation than rADAS cultured under steady condition.

本研究於第一階段將先評估脂肪來源之成體間葉幹細胞 (adipose-derived adult stem cells, ADAS) 之特性,並且觀察在加入生長因子(Epidermal Growth Factor, EGF、Platelet-derived Growth Factor, PDGF-BB) 之脂肪幹細胞 (ADAS-Growth factors, ADAS-GFs)以及ADAS於物理刺激之下,對於血管內皮細胞分化之可行性。
實驗初期先以牛頸動脈內皮細胞(bovine carotid artery endothelial cells, BEC)做為物理刺激之測試細胞,發現當頻率為1 Hz時,對於該細胞之增殖效率最好。再將BEC置換成大鼠脂肪幹細胞(rat ADAS, rADAS)及有加入生長因子之大鼠脂肪幹細胞(rat ADAS-GFs, rADAS-GFs),發現到在靜態培養時,有加入生長因子之幹細胞比起沒加入的幹細胞會有內皮相關基因產生。而在加入動態培養後,則更增加了CD31及CD34的表現量,顯示於生長因子以及動態培養環境之下,對於內皮分化有加成的效果。
其他識別: U0005-2008201017120600
Appears in Collections:生醫工程研究所

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