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The effect of dynamic and static conditions on the adipose-derived adult stem cells
|關鍵字:||脂肪幹細胞;adipose-derived adult stem cells (ADAS);牛頸動脈內皮細胞;EGF;PDGF-BB;bovine carotid artery endothelial cells (BEC);EGF;PDGF-BB||出版社:||生醫工程研究所||引用:|| Cao Y, Meng Y, Sun Z, Liao LM, Han Q, Li J, Liu YN, Zhao CH. Potential of human adipose tissue derived adult stem cells differentiate into endothelial cells. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2005;27(6):678-682.  Cao Y, Sun Z, Liao L, Meng Y, Han Q, Zhao RC. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun 2005;332(2):370-379.  Austin G. Smith. Embryo-devrived stem cells: of mice and men. Annual review of cell and developmental biology 2001; 17:435-462.  Caplan AI. Review: mesenchymal stem cells: cell-based reconstructive therapy in orthopedics. Tissue Eng 2005; 11:1998-1221.  Améen C, Strehl R, Björquist P, Lindahl A, Hyllner J, Sartipy P. Review: Human embryonic stem cells: current technologies and emerging industrial applications. Crit Rev Oncol Hematol 2008; 65: 54-80.  台灣牙醫界，2005年24卷3期14-18。  Tuan RS, Boland G, Tuli R. Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Res Ther 2003; 5(1): 32-45.  Zuk, PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz P, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell -based therapies. Tissue Eng, 2001, 7:211-223.  Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH. Human adipose tissue is a source of multi- potent stem cells. Mol Biol Cell, 2002,13:4279-4295.  Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7:211-228.  Nakagami H, Morishita R, Maeda K, Kikuchi Y, Ogihara T, Kaneda Y. Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy. J Atheroscler Thromb, 2006,13:77-81.  Rajashekhar G, Traktuev DO, Roell WC, Johnstone BH, Merfeld-Clauss S, Van Natta B, Rosen ED, March KL, Clauss M. IFATS collection: Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: role of canonical Wnt signaling. Stem Cells 2008;26(10):2674-2681.  Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, Tamarat R, Clergue M, Manneville C, Saillan-Barreau C, Duriez M, Tedgui A, Levy B, Penicaud L, Casteilla L. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation 2004;109(5):656-663.  Rupnick MA, Panigrahy D, Zhang CY, Dallabrida SM, Lowell BB, Langer R, Folkman MJ. Adipose tissue mass can be regulated through the vasculature. Proc Natl Acad Sci U S A 2002;99(16):10730-10735.  陳 兵，張柏根，張 建等 組織工程化血管構建的初步實驗研究 中華外 科雜誌，2005，19:1271-1274.  Vunjak-Novakovic G,Martin I,Obradovic B, Treppo S, Grodzinsky AJ, Langer R, Freed LE. Bioreactor conditions modulate the composition and mechanical properties of tissueengineered cartilage. J Orthop Res,1999,17:130-138.  Thompson CA, Colon-Hernandez P, Pomerantseva I, MacNeil BD, Nasseri B, Vacanti JP, Oesterle SN. A novel pulsatile, laminar flow bioreactor for the development of tissue engineered vascular structures. Tissue Eng, 2002, 8:1083-1088.  Kurpinski K, Chu J, Hashi C, Li S. Anisotropic mechanosensing by mesenchymal stem cells. Proc Natl Acad Sci U S A, 2006,103:16095-16100.  Solan A, Mitchell S, Moses M, Niklason L. Effect of pulse rate on collagen deposition in the tissue-engineered blood vessel. Tissue Eng, 2003, 9:579-586.  Kurpinski K, Chu J, Hashi C, Li S. Anisotropic mechanosensing by mesenchymal stem cells. Proc Natl Acad Sci U S A 2006;103(44):16095-16100.  Park JS, Chu JS, Cheng C, Chen F, Chen D, Li S. Differential effects of equiaxial and uniaxial strain on mesenchymal stem cells. Biotechnol Bioeng 2004;88(3):359-368.  Jennifer S. Park, Julia S.F. Chu, Catherine Cheng, Fanqing Chen, David Chen, Song Li. Differential Effects of Equiaxial and Uniaxial Strain on Mesenchymal Stem Cells. Biotechnology and Bioengineering. 2004 Nov 5;88(3):359-368  Chang JC, Su HL, Hsu SH. The use of peptide-delivery to protect human adipose-derived adult stem cells from damage caused by the internalization of quantum dots. Biomaterials. 2008 Mar;29(7):925-936.  Lei L, Liao W, Sheng P, Fu M, He A, Huang G. Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture. Sci China C Life Sci. 2007 Jun;50(3):320-328.  Kolf CM, Cho E, Tuan RS. Review: Biology of adult mesenchymal stem cells regulation of niche, self-renewal and differentiation. Arthritis Res Ther 2007; 9: 204.||摘要:||
In this study, human and rat adipose-derived adult stem cells (ADAS) were characterized. Next, the feasibility of endothelial differentiations of human and rat ADAS and growth factors [ epidermal growth factor (EGF) and platelet-derived growth factor (PDGF-BB) ] treated ADAS (ADAS-GFs) cultured under dynamic conditions were examined. For parameter identification, bovine carotid arterial endothelial cells (BEC) were first cultured under dynamic condition. The result showed that cell proliferation was better for BEC when cultured with dynamic strains at 1 Hz. Next, rat ADAS (rADAS) and rat ADAS-GFs (rADAS-GFs) were tested in the same condition. By comparing the expressions of endothelial marker genes from rADAS and rADAS-GFs cultured under steady condition, rADAS-GFs had higher endothelial differentiation ability. When rADAS-GFs were cultured under dynamic condition, the expression of CD31 and CD34 genes were enhanced, which indicated an increase in the endothelial differentiation. Therefore, rADAS-GFs cultured under dynamic condition showed more endothelial differentiation than rADAS cultured under steady condition.
本研究於第一階段將先評估脂肪來源之成體間葉幹細胞 (adipose-derived adult stem cells, ADAS) 之特性，並且觀察在加入生長因子(Epidermal Growth Factor, EGF、Platelet-derived Growth Factor, PDGF-BB) 之脂肪幹細胞 (ADAS-Growth factors, ADAS-GFs)以及ADAS於物理刺激之下，對於血管內皮細胞分化之可行性。
實驗初期先以牛頸動脈內皮細胞(bovine carotid artery endothelial cells, BEC)做為物理刺激之測試細胞，發現當頻率為1 Hz時，對於該細胞之增殖效率最好。再將BEC置換成大鼠脂肪幹細胞(rat ADAS, rADAS)及有加入生長因子之大鼠脂肪幹細胞(rat ADAS-GFs, rADAS-GFs)，發現到在靜態培養時，有加入生長因子之幹細胞比起沒加入的幹細胞會有內皮相關基因產生。而在加入動態培養後，則更增加了CD31及CD34的表現量，顯示於生長因子以及動態培養環境之下，對於內皮分化有加成的效果。
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