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標題: Novel G-quadruplex DNA Recognizers: Asymmetric Organic Compounds
作者: 陳俊瑋
Chen, Jyun-Wei
關鍵字: 端粒;telomere;G-四股結構;共振衍生物;染色體;G-quadruplex;naphthalene-vinylpyridine conjugates;chromosome
出版社: 生醫工程研究所
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Single-stranded telomeric DNA tends to form a four-base-paired planar structure termed G-quadruplex. We have synthesized a series of naphthalene-vinylpyridine conjugates which can recognize specific quadruplex structure, essentially the quadruplex structure of human telomeric sequence d(T2AG3)4. The fluorescence intensity of these conjugates increases apparently upon interacting with various DNAs and this property can be used to discriminate between duplex and quadruplex DNAs. Together with analysis of spectrum and experiments about electrophoresis, we screen a compound - IIb, which present sensitivity and binding preference to quadruplex d(T2AG3)4 over duplex DNA. Furthermore, staining of compound IIb on the metaphase chromosomes bright fluorescence spots at telomere-proximal regions were revealed. Base on these results, we demonstrated that the presence of quadruplex structures in human telomeres with Flu. staining method.
其他識別: U0005-1608201100212600
Appears in Collections:生醫工程研究所

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