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The influence of scaffolds of different nano-hemisphere arrays on skin tissue regeneration
|關鍵字:||低成本大量製造;Nanostructured scaffold;奈米模具;奈米結構支架;大鼠纖維母細胞;第一型膠原蛋白;Nanomolding;PLGA;Mouse fibroblast cell;Type I collagen||出版社:||生醫工程研究所||引用:|| C. R. Martin, "Nanomaterials: A Membrane-Based Synthetic Approach," Science, vol. 266, pp. 1961-1966, 1994.  Y. Yamauchi, N. Suzuki, L. Radhakrishnan, and L. Wang, "Breakthrough and future: nanoscale controls of compositions, morphologies, and mesochannel orientations toward advanced mesoporous materials," The Chemical Record, vol. 9, pp. 321-339, 2009.  B. Y. Kim, J. T. Rutka, and W. C. Chan, "Nanomedicine," New England Journal of Medicine, vol. 363, pp. 2434-2443, 2010.  R. Subbiah, M. Veerapandian, and K. S Yun, "Nanoparticles: functionalization and multifunctional applications in biomedical sciences," Current medicinal chemistry, vol. 17, pp. 4559-4577, 2010.  G. Cao and D. Liu, "Template-based synthesis of nanorod, nanowire, and nanotube arrays," Advances in Colloid and Interface Science, vol. 136, pp. 45-64, 2008.  G. J. Wang and H. T. 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本研究提出一套能簡單且低成本地大量製造奈米結構支架的方法，並應用其奈米結構支架影響纖維母細胞增生和第一型蛋白質分泌，進而縮短傷口修復時間且達美化傷口效果。製程乃是以陽極氧化鋁膜背阻障層高規則性的奈米半球結構陣列作為電鑄模板，電鑄成形奈米半球結構陣列鎳模具。利用可重複使用的奈米結構鎳模具大量翻印FDA認可之生醫材料聚乳酸甘醇酸(poly(lactic-co-glycolic acid, PLGA))、聚乳酸(polylactide, PLA))和天然材料甲殼素(Chitosan)奈米結構支架，製造材料表面具有均勻分佈且大小一致之奈米半球結構陣列。細胞培養以大鼠纖維母細胞(L929)培養於奈米結構支架表面，長時間細胞培養可觀察細胞在第二、三天時，受到支架表面奈米結構影響而大量增生，並從不同大小奈米結構對細胞增生影響得知纖維母細胞喜好生長於118 nm直徑大小半球結構上。利用酵素免疫分析，檢測培養第二天支架上纖維母細胞之第一型膠原蛋白分泌量，由其第一型膠原蛋白分泌量佐證奈米結構對纖維母細胞影響，並進一步分析奈米結構支架對縮短傷口癒合時間和美化傷口的可能性。
In this study, we demonstrate a relatively easy and cost effective method for the fabrication of nanostructured scaffolds, to shorten the time a wound takes to heal. Various scaffolds consisting of nanohemisphere arrays of poly(lactic-co-glycolic acid) (PLGA), polylactide (PLA), and chitosan were fabricated by casting using a nickel (Ni) replica mold. The Ni replica mold is electroformed using the highly ordered nanohemisphere array of the barrier-layer surface of an anodic aluminum oxide (AAO) membrane as the template. Mouse fibroblast cells (L929s) were cultured on the nanostructured polymer scaffolds to investigate the effect of these different nanohemisphere arrays on cell proliferation. The concentration of collagen type I on each scaffold was then measured through ELISA to find the most effective scaffold for shortening the wound healing process. The experimental data indicates that the proliferation of L929 is superior when a nanostructured PLGA scaffold with a feature size of 118 nm is utilized.
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