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Mechanism of near-infrared fluorophore, diglucosamid-SIDAG, for lesion malignancy screening
Lau, Jia -Yu
|關鍵字:||近紅外光螢光物;Diglucosamid-SIDAG;乳癌;組織與細胞階層吸收;breast cancer;tissue and cells uptake||出版社:||生醫工程研究所||引用:||A. Jemal, R. Siegel, E. Ward, Y. Hao, J. Xu, and M. J. Thun, "Cancer statistics, 2009," CA: a cancer journal for clinicians, vol. 59, pp. 225-249, 2009. F. A. Mettler, A. C. Upton, C. A. Kelsey, R. N. Ashby, R. D. Rosenberg, and M. N. Linver, "Benefits versus risks from mammography: A critical reasessment," Cancer, vol. 77, pp. 903-909, 1996. W. A. Berg, L. Gutierrez, M. S. NessAiver, W. B. Carter, M. Bhargavan, R. S. Lewis, and O. B. Ioffe, "Diagnostic Accuracy of Mammography, Clinical Examination, US, and MR Imaging in Preoperative Assessment of Breast Cancer1," Radiology, vol. 233, pp. 830-849, 2004. M. Kriege, C. T. M. Brekelmans, C. Boetes, P. E. Besnard, H. M. Zonderland, I. M. Obdeijn, R. A. Manoliu, T. Kok, H. Peterse, and M. M. A. 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判斷的長度長2.62×10-2 公分，偏差範圍±6.02×10-1 公分；惡性腫瘤螢光影像判斷的長度比超音波影像判斷的長度短0.05 公分，偏差範圍±2.27×10-1 公分。
最後在diglucosamid-SIDAG 的細胞階層吸收實驗中，經流氏細胞儀判讀得知，MCF-7細胞於作用1-8 小時之間，螢光物進入細胞的數量隨作用時間延長而增加，與裸鼠模型惡性植入物的螢光顯影趨勢雷同。
The aims of the research included three directions: verification of a nude mice xenograft model in representing both the clinical benign and malignant breast lesions, characterization of applying the diglucosamid-SIDAG contrasted imaging in delineation of lesion boundary with the nude mice xenograft model, and investigation of the mechanism for lesion malignancy screening the diglucosamid-SIDAG contrasted imaging.
The benign xenograft in the animal model resembled fibroadenoma with no evident neovascularization in vivo, high protein fibrosis with solid core and cleft structure in histological analysis, larger transverse diameter (parallel to the skin layers) than vertical diameter and central homogeneity under sonogram, and lower permeability and no noticeable clearance pattern of contrast agent in dynamic computer tomography scan. The malignant xenograft appeared with visible neovascularization in vivo, cell polymorphism and mitosis in histological analysis, greater vertical diameter (penetrating the architecture of skin layers) and ill-defined boundary under sonogram, and higher permeability and evident clearance pattern of contrast agent in dynamic computer tomography scan.
The accuracy of agreement analysis showed clinically acceptable bias of longitudinal axis length of lesion assessment between sonogram and calibrated fluorescence contrasted imaging. The in vitro cell lines study indicated that the preferential cellular uptake of the fluorophore in MCF-7 cell could be responsible for the “delayed wash-out phenomenon”.
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