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Fabrication of pillared 2D microvessel scaffold and hollow 3D scaffold on PLGA using femtosecond laser ablation
|關鍵字:||柱狀結構之PLGA人工微血管支架;Pillared PLGA microvessel scaffold;中空3D PLGA支架;飛秒雷射剝除系統;內皮細胞培養;hollow 3D PLGA scaffold;femtosecond laser ablation;Cell culture of endothelial cells||出版社:||生醫工程研究所||引用:||宋信文、梁晃千，“建立人類的人體工房－組織工程”，科學發展 Vol. 362, 6-11, 2003。 Madou MJ, Lee LJ, Daunert S, Lai Sa and Shih CH, “Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions.” Biomedical Microdevices, 3(3):245-254, 2003. Martin RS, Gawron AJ, Lunte SM and Henry CS, “Dual-Electrode Electrochemical Detection for Poly(dimethylsiloxane)-Fabricated Capillary Electrophoresis Microchips.” Analytical Chemistry, 72(14):3196-3202, 2000. Jiang G, Attiya S, Ocvirk G, Lee WE and Harrison DJ, “Red diode laser induced fluorescence detection with a confocal microscope on a microchip for capillary electrophoresis.” Biosensors and Bioelectronics, 14(10-11):861-869, 2000. 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The key issues involved in tissue engineering are how to culture specific cells on a suitable scaffold and to provide a satisfactory growth factor to regulate the differentiation and proliferation of the cells. Scaffolds function as the base for cell adhesion and migration, the place for the exchange of nutrients, and to deliver and retain cells and biochemical factors. In this study, the femtosecond laser ablation technique was implemented for the fabrication of 2D pillared microvessel scaffolds of polylactic-co-glycolic acid (PLGA) and hollow 3D PLGA scaffolds.
For the 2D pillared microvessel scaffolds, PLGA scaffolds consisting of 47 μm × 80 μm pillared branches were produced. Results of cell culturing of bovine endothelial cells (BECs) demonstrate that the cells adhere well and grow to surround each branch of the proposed pillared microvessel networks. This novel scaffold facilitates implementation of the conventional cell seeding process. The progress of cell growth can be observed in vitro by optical microscopy. The problems of becoming milky or completely opaque with the conventional PLGA scaffold after cell seeding can be resolved. In this study,
For the hollow 3D PLGA scaffolds, a salt ingot which was used as a temporary frame to define the shape of the desired scaffold was fabricated by extrusion molding. The salt ingot was then immersed in a PLGA solution and allowed to be entirely enveloped by the PLGA. The femtosecond laser ablation technique was used for direct writing of the desired pattern on the PLGA layer and finally the salt ingot inside was completely dissolved in distilled deionized water to obtain a hollow 3D PLGA scaffold on which BECs were then cultured. The cell culturing results are illustrated by SEM and fluorescent images and demonstrate that the BECs could adhere well and proliferate on the branches of the hollow 3D PLGA scaffold.
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