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dc.contributorGou-Jen Wangen_US
dc.contributor.authorWang, Hsiao-Weien_US
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dc.description.abstractThe key issues involved in tissue engineering are how to culture specific cells on a suitable scaffold and to provide a satisfactory growth factor to regulate the differentiation and proliferation of the cells. Scaffolds function as the base for cell adhesion and migration, the place for the exchange of nutrients, and to deliver and retain cells and biochemical factors. In this study, the femtosecond laser ablation technique was implemented for the fabrication of 2D pillared microvessel scaffolds of polylactic-co-glycolic acid (PLGA) and hollow 3D PLGA scaffolds. For the 2D pillared microvessel scaffolds, PLGA scaffolds consisting of 47 μm × 80 μm pillared branches were produced. Results of cell culturing of bovine endothelial cells (BECs) demonstrate that the cells adhere well and grow to surround each branch of the proposed pillared microvessel networks. This novel scaffold facilitates implementation of the conventional cell seeding process. The progress of cell growth can be observed in vitro by optical microscopy. The problems of becoming milky or completely opaque with the conventional PLGA scaffold after cell seeding can be resolved. In this study, For the hollow 3D PLGA scaffolds, a salt ingot which was used as a temporary frame to define the shape of the desired scaffold was fabricated by extrusion molding. The salt ingot was then immersed in a PLGA solution and allowed to be entirely enveloped by the PLGA. The femtosecond laser ablation technique was used for direct writing of the desired pattern on the PLGA layer and finally the salt ingot inside was completely dissolved in distilled deionized water to obtain a hollow 3D PLGA scaffold on which BECs were then cultured. The cell culturing results are illustrated by SEM and fluorescent images and demonstrate that the BECs could adhere well and proliferate on the branches of the hollow 3D PLGA scaffold.en_US
dc.description.tableofcontents誌謝 i 摘要 ii Abstract iii 目錄 iv 圖目錄 vi 表目錄 ix 第一章 緒論 1 1.1 研究背景與動機 1 1.2 研究目的 10 第二章 研究方法與材料 11 2.1 雷射簡介 11 2.1.1 雷射加工機制 11 2.1.2 飛秒雷射加工 11 2.2 PLGA配製 12 2.3 牛頸動脈內皮細胞 (BEC)培養 13 2.3.1 細胞製備 13 2.3.2 細胞植覆 14 2.3.3 螢光顯微鏡觀察 14 2.4 冷凍包埋切片 14 2.4.1 試片製備 14 2.4.2 儀器使用 14 2.4.3 Hematoxylin & Eosin (HE)染色 15 第三章 2D柱狀PLGA微血管支架製作與細胞培養 16 3.1 實驗流程圖 16 3.2 PLGA薄膜製備 16 3.3 飛秒雷射加工參數建立 16 3.3.1 能量與PLGA薄膜關係 17 3.3.2電性量測 23 3.4 柱狀PLGA支架設計 24 3.4.1 支架雛形 24 3.4.2 改良支架 25 3.5 柱狀PLGA支架之細胞培養 28 3.5.1 細胞植覆柱狀PLGA支架 28 3.5.2 冷凍包埋切片觀察 29 3.5.3 細胞螢光染色結果 30 3.5.4 細胞貼附率計算 31 第四章 中空3D PLGA支架製作與細胞培養 33 4.1 鹽錠壓製 33 4.2 PLGA包覆 34 4.3 飛秒雷射加工 35 4.3.1 飛秒雷射加工參數建立 35 4.3.2 簡易加工測試可行性 35 4.3.3 雷射加工 37 4.3.4 雷射加工參數最佳化 38 4.4 中空3D半球PLGA支架設計製作 39 4.4.1 球體PLGA支架之構想與架構 39 4.4.2 半球鹽錠設計製作 40 4.4.3 簡易加工測試可行性 42 4.5 細胞植覆中空3D PLGA支架 43 4.5.1 細胞植覆 43 4.5.2 細胞貼附率計算 47 第五張 結論與未來展望 49 5.1 結論 49 5.2 未來展望 49 參考文獻 51zh_TW
dc.subjectPillared PLGA microvessel scaffolden_US
dc.subject中空3D PLGA支架zh_TW
dc.subjecthollow 3D PLGA scaffolden_US
dc.subjectfemtosecond laser ablationen_US
dc.subjectCell culture of endothelial cellsen_US
dc.title利用飛秒雷射製作2D柱狀PLGA微血管支架與中空3D PLGA支架zh_TW
dc.titleFabrication of pillared 2D microvessel scaffold and hollow 3D scaffold on PLGA using femtosecond laser ablationen_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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