Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40156
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dc.contributor.authorTsai, Jaw-Jien_US
dc.contributor.authorLiu, Shing-Hwaen_US
dc.contributor.authorYin, Sui-Chuen_US
dc.contributor.authorYang, Cheng-Ningen_US
dc.contributor.authorHsu, Hong-Shengen_US
dc.contributor.authorChen, Wen-Baoen_US
dc.contributor.authorLiao, En-Chihen_US
dc.contributor.authorLee, Wen-Janeen_US
dc.contributor.authorPan, Hung-Chuanen_US
dc.contributor.authorSheu, Meei-Lingen_US
dc.contributor.other國立中興大學生物醫學研究所zh_TW
dc.contributor.otherNational Chung Hsing University,Graduate Institute of Biomedical Sciencesen_US
dc.date2011-9zh_TW
dc.date.accessioned2014-06-06T08:03:17Z-
dc.date.available2014-06-06T08:03:17Z-
dc.identifier.issn1932-6203zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40156-
dc.description.abstractBackground: Allergic disease can be characterized as manifestations of an exaggerated inflammatory response to environmental allergens triggers. Mite allergen Der-p2 is one of the major allergens of the house dust mite, which contributes to TLR4 expression and function in B cells in allergic patients. However, the precise mechanisms of Der-p2 on B cells remain obscure. Methodology/Principal Findings: We investigated the effects of Der-p2 on proinflammatory cytokines responses and Toll-like receptor-4 (TLR4)-related signaling in human B cells activation. We demonstrated that Der-p2 activates proinflammatory cytokines, TLR4 and its co-receptor MD2. ERK inhibitor PD98059 significantly enhanced TLR4/MD2 expression in Der-p2-treated B cells. Der-p2 markedly activated mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) and decreased p38 phosphorylation in B cells. MKP-1-siRNA downregulated TLR4/MD2 expression in Der-p2-treated B cells. In addition, Der-p2 significantly up-regulated expression of co-stimulatory molecules and increased B cell proliferation. Neutralizing Der-p2 antibody could effectively abrogate the Der-p2-induced B cell proliferation. Der-p2 could also markedly induce NF-kappa B activation in B cells, which could be counteracted by dexamethasone. Conclusions/Significance: These results strongly suggest that Der-p2 is capable of triggering B cell activation and MKP-1-activated p38/MAPK dephosphorylation-regulated TLR4 induction, which subsequently enhances host immune, defense responses and development of effective allergic disease therapeutics in B cells.en_US
dc.language.isoen_USzh_TW
dc.relationPlos One, Volume 6, Issue 9, Page(s) e23249.en_US
dc.subjectmap kinase phosphatase-1en_US
dc.subjectinnate immune-responsesen_US
dc.subjectproteinen_US
dc.subjecttlr4en_US
dc.subjectlungen_US
dc.subjectmiceen_US
dc.subjectlipopolysaccharideen_US
dc.subjectinfectionen_US
dc.subjectmediatoren_US
dc.subjectcellsen_US
dc.titleMite Allergen Der-p2 Triggers Human B Lymphocyte Activation and Toll-Like Receptor-4 Inductionen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1371/journal.pone.0023249zh_TW
dc.contributor.catalogerMiao-zhen Luoen_US
item.grantfulltextrestricted-
item.fulltextwith fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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