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|標題:||An improved PCR method for gender identification of eagles||作者:||Chang, H.W.
|關鍵字:||Gender identification;Eagles;Spilornis cheela hoya;Falconiformes;CHD;gene;Gender-specific primers;sex identification;avian chd;birds;genes||Project:||Molecular and Cellular Probes||期刊/報告no：:||Molecular and Cellular Probes, Volume 22, Issue 3, Page(s) 184-188.||摘要:||
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay. (C) 2008 Elsevier Ltd. All rights reserved.
|Appears in Collections:||生物醫學研究所|
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