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標題: An improved PCR method for gender identification of eagles
作者: Chang, H.W.
Chou, T.C.
Gu, D.L.
Cheng, C.A.
Chang, C.C.
Yao, C.T.
Chuang, L.Y.
Wen, C.H.
Chou, Y.C.
Tan, K.Y.
Cheng, C.C.
關鍵字: Gender identification;Eagles;Spilornis cheela hoya;Falconiformes;CHD;gene;Gender-specific primers;sex identification;avian chd;birds;genes
Project: Molecular and Cellular Probes
期刊/報告no:: Molecular and Cellular Probes, Volume 22, Issue 3, Page(s) 184-188.
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay. (C) 2008 Elsevier Ltd. All rights reserved.
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2007.12.004
Appears in Collections:生物醫學研究所

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