Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40278
DC FieldValueLanguage
dc.contributor.authorHo, T.F.en_US
dc.contributor.author張嘉哲zh_TW
dc.contributor.authorPeng, Y.T.en_US
dc.contributor.authorChuang, S.M.en_US
dc.contributor.authorLin, S.C.en_US
dc.contributor.authorFeng, B.L.en_US
dc.contributor.authorLu, C.H.en_US
dc.contributor.authorYu, W.J.en_US
dc.contributor.authorChang, J.S.en_US
dc.contributor.authorChang, C.C.en_US
dc.contributor.author莊秀美zh_TW
dc.date2009zh_TW
dc.date.accessioned2014-06-06T08:03:32Z-
dc.date.available2014-06-06T08:03:32Z-
dc.identifier.issn0041-008Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40278-
dc.description.abstractProdigiosin is a bacterial metabolite with potent anticancer activity, which is attributed to its proapoptotic effect selectively active in malignant cells. Still, the molecular mechanisms whereby prodigiosin induces apoptosis remain largely unknown. In particular, the role of suivivin, a vital inhibitor of apoptosis, in prodigiosin-induced apoptosis has never been addressed before and hence was the primary goal of this study. Our results showed that prodigiosin close-dependently induced down-regulation of survivin in multiple breast carcinoma cell lines, including MCF-7, T-47D and MDA-MB-231. This down-regulation is mainly regulated at the level of transcription, as prodigiosin reduced the levels of both survivin mRNA and survivin promoter activity but failed to rescue survivin expression when proteasome-mediated degradation is abolished. Importantly, overexpression of survivin rendered cells more resistant to prodigiosin, indicating all essential role of survivin down-regulation in prodigiosin-induced apoptosis. In addition, we found that prodigiosin synergistically enhanced cell death induced by paclitaxel, a chemotherapy drug known to up-regulate survivin that ill turn confers its own resistance. This paclitaxel sensitization effect of prodigiosin is ascribed to the lowering of survivin expression, because prodigiosin was shown to counteract survivin induction by paclitaxel and, notably, the sensitization effect was severely abrogated in cells that overexpress survivin. Taken together, our results argue that down-regulation of survivin is all integral component mediating prodigiosin-induced apoptosis in human breast cancer cells, and further suggest the potential of prodigiosin to sensitize anticancer drugs, including paclitixel, in the treatment of breast cancer. (C) 2008 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationToxicology and Applied Pharmacologyen_US
dc.relation.ispartofseriesToxicology and Applied Pharmacology, Volume 235, Issue 2, Page(s) 253-260.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.taap.2008.12.009en_US
dc.subjectProdigiosinen_US
dc.subjectSurvivinen_US
dc.subjectPaclitaxelen_US
dc.subjectApoptosisen_US
dc.subjectBreast canceren_US
dc.subjectanticancer agent prodigiosinen_US
dc.subjectlung-cancer cellsen_US
dc.subjectinduced apoptosisen_US
dc.subjecttargeting survivinen_US
dc.subjectmicrotubule dynamicsen_US
dc.subjectgene-expressionen_US
dc.subjectovarian-canceren_US
dc.subjecttumor-cellsen_US
dc.subjectresistanceen_US
dc.subjecttaxolen_US
dc.titleProdigiosin down-regulates survivin to facilitate paclitaxel sensitization in human breast carcinoma cell linesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.taap.2008.12.009zh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
Appears in Collections:生物醫學研究所
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.