Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40281
DC FieldValueLanguage
dc.contributor.authorHuang, S.W.en_US
dc.contributor.author張嘉哲zh_TW
dc.contributor.authorLiu, K.T.en_US
dc.contributor.authorChang, C.C.en_US
dc.contributor.authorChen, Y.J.en_US
dc.contributor.authorWu, C.Y.en_US
dc.contributor.authorTsai, J.J.en_US
dc.contributor.authorLu, W.C.en_US
dc.contributor.authorWang, Y.T.en_US
dc.contributor.authorLiu, C.M.en_US
dc.contributor.authorShieh, J.J.en_US
dc.contributor.author謝政哲zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:03:32Z-
dc.date.available2014-06-06T08:03:32Z-
dc.identifier.issn0007-0963zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40281-
dc.description.abstractP>Background Imiquimod shows antitumour activity through the stimulation of cell-mediated immunity in vivo. Recent studies have shown that imiquimod promotes apoptosis in melanoma cells and induces autophagy in macrophage cell lines. Objectives To evaluate the imiquimod-induced apoptosis, autophagy and their relationship in a basal cell carcinoma (BCC) cell line. Methods Cell viability was determined by XTT test. Apoptosis was evaluated by DNA content assay, annexin V/propidium iodide staining assay and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling assay. Autophagy was determined by LC3 immunoblotting, EGFP-LC3 puncta formation and quantification of acidic vesicular organelles with acridine orange staining. The temporal and spatial differences of imiquimod-induced apoptosis and autophagy were examined by immunoblotting and simultaneously monitored by staining the EGFP-LC3 transfected cells with caspase 3 fluorogenic substrate. We inhibited the apoptosis and autophagy by pancaspase inhibitor and siRNA for Beclin 1 or Atg5, respectively, to evaluate the interplay between imiquimod-induced apoptosis and autophagy. Results We found that imiquimod induces autophagy and apoptosis in BCC cells in a time- and dose-dependent manner. Imiquimod not only induced EGFP-LC3 puncta formation for autophagy, but also simultaneously activated an apoptotic caspase cascade in the same cells. Both apoptosis and autophagy induced by imiquimod cooperate to cause BCC cell death. However, inhibition of imiquimod-induced apoptosis increased the strength of autophagy, and inhibition of imiquimod-induced autophagy further promoted cell apoptosis. Conclusions This study not only demonstrates that imiquimod can directly induce autophagy and apoptosis in BCC cells, but also shows the cooperation and coordination between these two processes to induce cell death.en_US
dc.language.isoen_USzh_TW
dc.relationBritish Journal of Dermatologyen_US
dc.relation.ispartofseriesBritish Journal of Dermatology, Volume 163, Issue 2, Page(s) 310-320.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1365-2133.2010.09827.xen_US
dc.subjectapoptosisen_US
dc.subjectautophagyen_US
dc.subjectbasal call carcinomaen_US
dc.subjectimiquimoden_US
dc.subjectimmune-response modifieren_US
dc.subjectmalignant-melanomaen_US
dc.subjectdeathen_US
dc.subjectcanceren_US
dc.subjectpathwaysen_US
dc.subjectsurvivalen_US
dc.subjecttlr7en_US
dc.titleImiquimod simultaneously induces autophagy and apoptosis in human basal cell carcinoma cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1365-2133.2010.09827.xzh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
Appears in Collections:生物醫學研究所
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