Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40305
DC FieldValueLanguage
dc.contributor.authorWu, C.T.en_US
dc.contributor.author許美鈴zh_TW
dc.contributor.authorSheu, M.L.en_US
dc.contributor.authorTsai, K.S.en_US
dc.contributor.authorWeng, T.I.en_US
dc.contributor.authorChiang, C.K.en_US
dc.contributor.authorLiu, S.H.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:03:34Z-
dc.date.available2014-06-06T08:03:34Z-
dc.identifier.issn1096-6080zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40305-
dc.description.abstractContrast medium (CM) induces a direct toxic effect on renal tubular cells. This toxic effect may have a role in the pathophysiology of CM-induced nephropathy. CM has been shown to affect the endoplasmic reticulum (ER) related capacity. Unfolded protein response (UPR) is known as a prosurvival response to reduce the accumulation of unfolded proteins and restore normal ER function. However, the role of ER stress related UPR in the CM-induced renal cell injury still remains unclear. In this study, we examined whether UPR participates in urografin (an ionic CM)-induced renal tubular cells apoptosis. Treatment with urografin in normal rat renal tubular cell line (NRK52E) markedly increased cell apoptosis and decreased cell viability with a dose- and time-dependent manner. The cell necrosis was not increased in urografin-treated cells. Urografin also enhance the induction of ER stress related markers in NRK52E cells, including glucose-regulated protein (GRP)78 and GRP94 expressions, procaspase-12 cleavage, phosphorylation of PERK (PKR [double-stranded RNA activated protein kinase]-like ER kinase), and eukaryotic initiation factor 2 alpha (eIF2 alpha). Salubrinal, a selective inhibitor of eIF2 alpha dephosphorylation, effectively decreased urografin-induced cell apoptosis. Furthermore, transfection of GRP78-small interfering RNA in NRK52E cells significantly enhanced urografin-induced cell apoptosis. These results suggest that GRP78/eIF2 alpha-related signals play a protective role during UPR, and the activation of ER stress related UPR may play an important regulative role in urografin-induced renal tubular injury.en_US
dc.language.isoen_USzh_TW
dc.relationToxicological Sciencesen_US
dc.relation.ispartofseriesToxicological Sciences, Volume 114, Issue 2, Page(s) 295-301.en_US
dc.relation.urihttp://dx.doi.org/10.1093/toxsci/kfq006en_US
dc.subjectcontrast mediumen_US
dc.subjectER stressen_US
dc.subjectunfolded protein responseen_US
dc.subjectapoptosisen_US
dc.subjectradiographic contrast-mediaen_US
dc.subjectinduced nephropathyen_US
dc.subjectinduced apoptosisen_US
dc.subjecttumor-developmenten_US
dc.subjectn-acetylcysteineen_US
dc.subjectoxidant stressen_US
dc.subjectllc-pk1 cellsen_US
dc.subjecteren_US
dc.subjectstressen_US
dc.subjectanalogen_US
dc.subjectproliferationen_US
dc.titleThe Role of Endoplasmic Reticulum Stress-Related Unfolded Protein Response in the Radiocontrast Medium-Induced Renal Tubular Cell Injuryen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1093/toxsci/kfq006zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:生物醫學研究所
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