Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40356
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dc.contributor.authorChen, H.W.en_US
dc.contributor.author陳健尉zh_TW
dc.contributor.authorChen, J.J.W.en_US
dc.contributor.authorYu, S.L.en_US
dc.contributor.authorLi, H.N.en_US
dc.contributor.authorYang, P.C.en_US
dc.contributor.authorSu, C.M.en_US
dc.contributor.authorAu, H.K.en_US
dc.contributor.authorChang, C.W.en_US
dc.contributor.authorChien, L.W.en_US
dc.contributor.authorChen, C.S.en_US
dc.contributor.authorTzeng, C.R.en_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T08:03:39Z-
dc.date.available2014-06-06T08:03:39Z-
dc.identifier.issn0268-1161zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40356-
dc.description.abstractBACKGROUND: Hatching is an important process for early embryo development, differentiation and implantation. However, little is known about its regulatory mechanisms. By integrating the technologies of RNA amplification and cDNA microarrays, it has become possible to study the gene expression profile at this critical stage. METHODS: Pre-hatched and hatched ICR mouse embryos (25 blastocysts in each group were used in the triplicate experiments) were collected for RNA extraction, amplification, and microarray analysis (the mouse cDNA microarray, 6144 genes, including expressed sequence tags). RESULTS: According to cDNA microarray data, we have identified 85 genes that were expressed at a higher level in hatched blastocyst than in pre-hatched blastocysts. In this study, 47 hatching-related candidate genes were verified via re-sequencing. Some of these genes have been selected and confirmed by real-time quantitative RT-PCR. These hatching-specific genes were also expressed at a lower level in the delayed growth embryos (morula or blastocyst without hatching at day 6 post hCG). These genes included: cell adhesion and migration molecules [E-cadherin, neuronal cell adhesion molecule (NCAM), lectin, galactose binding, soluble 7 (Lgals7), vanin 3 and biglycan], epigenetic regulators (Dnmt1, and SIN3 yeast homolog A), stress response regulators (heme oxygenase 1) and immunoresponse regulators [interleukin (IL)-2-inducible T-cell kinase, IL-4R, interferon-gamma receptor 2, and neurotrophin]. The immunostaining of E-cadherin and NCAM showed strong and specific localization in hatched blastocyst. CONCLUSIONS: This work provides important information for studying the mechanisms of blastocyst hatching and implantation. These hatching-specific genes may have potential as new drug targets for controlling fertility.en_US
dc.language.isoen_USzh_TW
dc.relationHuman Reproductionen_US
dc.relation.ispartofseriesHuman Reproduction, Volume 20, Issue 9, Page(s) 2492-2501.en_US
dc.relation.urihttp://dx.doi.org/10.1093/humrep/dei084en_US
dc.subjectblastocysten_US
dc.subjectcDNA microarrayen_US
dc.subjectgene expressionen_US
dc.subjecthatchingen_US
dc.subjectimplantationen_US
dc.subjectsuppression subtractive hybridizationen_US
dc.subjectdifferentially expressed genesen_US
dc.subjectin-vitroen_US
dc.subjectpreimplantation developmenten_US
dc.subjectmammalian developmenten_US
dc.subjectembryoen_US
dc.subjectdevelopmenten_US
dc.subjectdna methylationen_US
dc.subjectgrowth-factorsen_US
dc.subjectmessenger-rnaen_US
dc.subjectnitric-oxideen_US
dc.titleTranscriptome analysis in blastocyst hatching by cDNA microarrayen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1093/humrep/dei084zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
Appears in Collections:生物醫學研究所
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