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|標題:||Tumor-associated macrophages: The double-edged sword in cancer progression||作者:||Chen, J.J.W.
|關鍵字:||cell lung-cancer;nf-kappa-b;human breast-cancer;growth-factor;patient survival;epithelial-cells;messenger-rna;endothelial-cells;kinase-activity;expression||Project:||Journal of Clinical Oncology||期刊/報告no：:||Journal of Clinical Oncology, Volume 23, Issue 5, Page(s) 953-964.||摘要:||
Purpose Inflammation plays a critical role in cancer progression. In this study we investigate the protumorigenic activities and gene expression profiles of lung cancer cells after interaction with macrophages. Materials and Methods We measured intratumoral microvessel counts and macrophage density in 41 lung cancer tumor specimens and correlated these with the patients' clinical outcome. The interaction between macrophages and cancer cell lines was assessed using a transwell coculture system. The invasive potential was evaluated by in vitro invasion assay. The matrix-degrading activity was assayed by gelatin zymography. The microarray was applied to a large-scale analysis of the genes involved in the interaction, as well as to monitor the gene expression profiles of lung cancer cells responding to anti-inflammatory drugs in cocultures. Results The macrophage density positively correlated with microvessel counts and negatively correlated with patient relapse-free survival (P <.05). After corculture with macrophages, lung cancer cell lines exhibited higher invasive potentials and matrix-degrading activities. We identified 50 genes by microarray that were upregulated more than two-fold in cancer cells after coculture. Northern blot analyses confirmed some gene expression such as interleukin-6, interleukin-8, and matrix metalloproteinase 9. The two-dimensional hierarchical clustering also demonstrated that the gene expression profiles of lung cancer cells responding to various anti-inflammatory drugs in cocultures are distinct. Conclusion The interaction of lung cancer cells and macrophages can promote the invasiveness and matrix-degrading activity of cancer cells. Our results also suggest that a great diversity of gene expression occurs in this interaction, which may assist us in understanding the process of cancer metastasis. (C) 2005 by American Society of Clinical Oncology.
|Appears in Collections:||生物醫學研究所|
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