Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40364
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dc.contributor.authorLiu, C.W.en_US
dc.contributor.author陳健尉zh_TW
dc.contributor.authorLin, C.C.en_US
dc.contributor.authorChen, J.J.W.en_US
dc.contributor.authorTseng, M.J.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:03:39Z-
dc.date.available2014-06-06T08:03:39Z-
dc.identifier.issn0721-7714zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40364-
dc.description.abstractThe objectives of this research were first to isolate plastid gene sequences from cabbage (Brassica oleracea L. var. capitata L.), and to establish the chloroplast transformation technology of Brassica. A universal transformation vector (pASCC201) for Brassica chloroplast was constructed with trnV-rrn16S (left) and trnI-trnA-rrn23S (right) of the IRA region as a recombination site for the transformed gene. In transforming plasmid pASCC201, a chimeric aadA gene was cloned between the rrn16S and rrn23S plastid gene borders. Expression of aadA confers resistance to spectinomycin and streptomycin antibiotics. The uidA gene was also inserted into the pASCC201 and transferred into the leaf cells of cabbage via particle gun mediated transformation. Regenerated plantlets were selected by 200 mg/l spectinomycin and streptomycin. After antibiotic selection, the regeneration percentage of the two cabbage cultivars was about 2.7-3.3%. The results of PCR testing and Southern blot analysis confirmed that the uidA and aadA genes were present in the chloroplast genome via homologously recombined. Northern blot hybridizations, immunoblotting and GUS histochemical assays indicated that the uidA gene were stable integrated into the chloroplast genome. Foreign protein was accumulated at 3.2-5.2% of the total soluble protein in transgenic mature leaves. These results suggest that the expression of a variety of foreign genes in the chloroplast genome will be a powerful tool for use in future studies.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Cell Reportsen_US
dc.relation.ispartofseriesPlant Cell Reports, Volume 26, Issue 10, Page(s) 1733-1744.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s00299-007-0374-zen_US
dc.subjectaadA geneen_US
dc.subjectbombardmenten_US
dc.subjectcabbageen_US
dc.subjectchloroplast transformationen_US
dc.subjectuidA geneen_US
dc.subjectcomplete nucleotide-sequenceen_US
dc.subjectgreen fluorescent proteinen_US
dc.subjectplant-baseden_US
dc.subjectvaccineen_US
dc.subjectplastid transformationen_US
dc.subjecttransgenic chloroplastsen_US
dc.subjecttobaccoen_US
dc.subjectchloroplastsen_US
dc.subjectconfers resistanceen_US
dc.subjectselectable markeren_US
dc.subjectgene organizationen_US
dc.subjectexpressionen_US
dc.titleStable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardmenten_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s00299-007-0374-zzh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生物醫學研究所
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