Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40371
DC FieldValueLanguage
dc.contributor.authorLee, C.Y.en_US
dc.contributor.author陳健尉zh_TW
dc.contributor.authorAgrawal, D.C.en_US
dc.contributor.authorWang, C.S.en_US
dc.contributor.authorYu, S.M.en_US
dc.contributor.authorChen, J.J.W.en_US
dc.contributor.authorTsay, H.S.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-06T08:03:40Z-
dc.date.available2014-06-06T08:03:40Z-
dc.identifier.issn0032-0943zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40371-
dc.description.abstractThe study of functional genomics has paved the way for directed approaches to the generation of genetically modified plants that produce novel and/or improved yields of pharmaceuticals. In the present study, an activation tagging mutagenesis (ATM) population of Salvia miltiorrhiza Bunge, a medicinal plant, was established by Agrobacterium-mediated transformation. The optimum conditions for Agrobacterium transformation were determined by the expression of green fluorescent protein. Under these optimized conditions, we isolated 1435 ATM cell lines with our initial antibiotic selection. Of these 1435 ATM cell lines, six lines (T1 -T6) showed a red color on a selective medium containing 4.5 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), which is used as a phenotypic model system to identify the accumulation of tanshinones. 700 out of 1435 ATM cell lines were tested with a beta-glucuronidase (GUS) assay, 35 showed GUS activity. Southern blotting analysis revealed that the T1 -T7 ATM cell lines have a single copy of the T-DNA insertion. Comparative analysis by high-performance liquid chromatography of the tanshinones expressed by non-transformed and ATM-transformed calli revealed varying quantities of tanshinones. There were negligible tanshinones in non-transformed white calli induced with 2,4-D. ATM lines T1 -T6 showed significant increases in the yields of tanshinone-1(up to 43-fold), tanshinone-IIA (up to 26-fold) and cryptotanshinone (up to 104-fold) compared with those of the non-transgenic lines on 2,4-D medium. Interestingly, the yield of cryptotanshinone from line T4 on 2,4-D medium was two times higher than that of the non-transgenic lines on trans-zeatin riboside medium. To the best of our knowledge, this is the first report of a quantitative and qualitative improvement in quinoid diterpene production achieved in a medicinally important plant species by activation tagging.en_US
dc.language.isoen_USzh_TW
dc.relationPlanta Medicaen_US
dc.relation.ispartofseriesPlanta Medica, Volume 74, Issue 7, Page(s) 780-786.en_US
dc.relation.urihttp://dx.doi.org/10.1055/s-2008-1074527en_US
dc.subjectactivation taggingen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectgene trappingen_US
dc.subjectgreenen_US
dc.subjectfluorescent proteinen_US
dc.subjectSalvia miltiorrhizaen_US
dc.subjectLamiaceaeen_US
dc.subjecttanshinonesen_US
dc.subjectinsertional mutagenesisen_US
dc.subjecttransformationen_US
dc.subjectagrobacteriumen_US
dc.subjectculturesen_US
dc.subjectbungeen_US
dc.subjectriceen_US
dc.subjecttranscriptionen_US
dc.subjectarabidopsisen_US
dc.subjectcallusen_US
dc.subjecttissueen_US
dc.titleT-DNA activation tagging as a tool to isolate Salvia miltiorrhiza transgenic lines for higher yields of tanshinonesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1055/s-2008-1074527zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生物醫學研究所
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.